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Recombinant Human IgG1 Fc Protein, CF Best Seller

Bio-Techne includes R&D Systems | Catalog # 110-HG

Analyzed by SEC-MALS
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110-HG-100

Key Product Details

Source

NS0

Accession #

Structure / Form

Dimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IgG1 protein
IEGRMD Human IgG1
(Pro100-Lys330)
Substitutions: Leu117Ala, Leu118Glu, Gly120Ala, Thr276Ala
Accession # P01857.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile

Predicted Molecular Mass

26.6 kDa

SDS-PAGE

30-35 kDa, reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Human IgG1 Fc Protein, CF

Recombinant Human IgG1 Fc Protein SEC-MALS.

Recombinant Human IgG1/Fc Protein (Catalog # 110-HG) has a molecular weight (MW) of 55.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a dimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Formulation, Preparation and Storage

110-HG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

UniProt

Additional IgG1 Products

Product Documents for Recombinant Human IgG1 Fc Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IgG1 Fc Protein, CF

For research use only

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