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Recombinant Human IgG1 Fc Avi-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # AVI110

Biotinylated
Catalog #
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AVI110-050

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer, Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IgG1 protein
IEGRMD Human IgG1
(Pro100-Lys330)
Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile

Predicted Molecular Mass

28 kDa

SDS-PAGE

30-42 kDa

Activity

Measured by its binding ability in a functional ELISA.

When Biotinylated Recombinant Human IgG1 Fc Avi-tag (Catalog # AVI110) is immobilized at 2 µg/mL (100 µL/well) onto a Streptavidin coated plate (Catalog # CP004), it binds to Human C1q with an ED50 of 0.2-2.0 μg/mL.

Scientific Data Images for Recombinant Human IgG1 Fc Avi-tag Protein, CF

Recombinant Human IgG1 Fc Avi-tag Protein Binding Activity

Recombinant Human IgG1 Fc Avi-tag Protein Binding Activity

When Biotinylated Recombinant Human IgG1 Fc Avi-tag (Catalog # AVI110) is immobilized at 2 µg/mL (100 µL/well) onto a Streptavidin coated plate (Catalog # CP004), it binds to Human C1q with an ED50 of 0.2-2.0 µg/mL.
Recombinant Human IgG1 Fc Avi-tag Protein SDS-PAGE

Recombinant Human IgG1 Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human IgG1Fc Avi-tag (Catalog # AVI110) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-42 kDa and 60-80 kDa, respectively.

Formulation, Preparation and Storage

AVI110
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG1

Human Fc is the crystallizable fragment in the constant region of the human immunoglobulin heavy chain. Members of the immunoglobulin superfamily (IgSF) consist of two heavy (H) and two light (L) chains, with each component containing one variable (V) and one or more constant (C) domains (1, 2). Produced by B lymphocytes, Ig gamma (IgG) is the predominant isotype found in the body and has the longest half-life of all immunoglobulin isotypes (3). Four IgG subclasses (IgG1-4) were identified based on structural, antigenic and functional differences in the constant region of the heavy chain. The constant tail of the antibody is where proteins involved in immune responses typically bind, most notably the first component of the complement pathway, C1q, and Fc receptors found on immune cells such as B lymphocytes. Affinities for C1q and Fc gamma receptor (Fc gammaR) differ among the IgG subclasses. Fc receptors mediate the recruitment of immune cells to a site of infection (4). Engineered crystallizable fragment (Fc) regions of antibody which assume a unique and unprecedented asymmetric structure within the homodimeric Fc polypeptide, enable completely selective binding to the complement component C1q and activation of complement via the classical pathway without any concomitant engagement of the Fc gammaR (5).

References

  1. Williams, A.F. and A.N. Barclay (1988) Annu. Rev. Immunol. 6:381.
  2. Harpaz, Y. and C. Chothia (1994) J. Mol. Biol. 238:528.
  3. Schroeder, H.W. and L. Cavacini (2010) J. Allergy Clin. Immunol. 125:S41.
  4. Duncan, A.R. and G. Winter (1998) Nature 332:738.
  5. Lee, C.H. et al. (2017) Nat. Immunol. 18:889.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

UniProt

Additional IgG1 Products

Product Documents for Recombinant Human IgG1 Fc Avi-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IgG1 Fc Avi-tag Protein, CF

For research use only

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