Recombinant Human IGF-I/IGF-1, Animal-Free Protein

Name: Recombinant Human IGF-I/IGF-1, Animal-Free Protein
Brand: R&D Systems
SKU: AFL291

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AFL291

Also available, GMP IGF-I/IGF-1 Protein, Catalog # 291-GMP! Animal-free and manufactured under GMP guidelines for therapeutic manufacturing applications.

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Key Product Details

Learn more about Animal-Free Recombinant Proteins

Source:	E. coli
Accession #:	P05019.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IGF-I/IGF-1, Animal-Free Protein

Product Specifications for Recombinant Human IGF-I/IGF-1, Animal-Free Protein

Source	E. coli-derived human IGF-I/IGF-1 protein Gly49-Ala118 Produced using non-animal reagents in an animal-free laboratory.
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly49
Predicted Molecular Mass	7.6 kDa
Activity	Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 0.3-1.5 ng/mL. The specific activity of recombinant human IGF-I/IGF-1 is >1000 IU/mg, which is calibrated against the human IGF-I/IGF-1 WHO International Standard (NIBSC code: 91/554).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AFL291

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IGF-I/IGF-1

Insulin-like growth factor I (IGF-I or IGF-1), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I/IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I/IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF‑II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I/IGF-1 binds IGF‑I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half‑life and modulates its interactions with receptors (10).

Item	Value
References	Philippou, A. et al. (2007) In Vivo 21:45. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275. Malemud, C.J. (2007) Clin. Chim. Acta 375:10. Samani, A.A. et al. (2007) Endocrine Rev. 28:20. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
Long Name	Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs	3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names	IBP1, IGF-1, IGF-I, IGF-IA, IGF-IB, IGF1, IGF1A, IGFI, MGF, Mechano growth factor, Somatomedin A, Somatomedin C, insulin-like growth factor 1, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor I, insulin-like growth factor IA, insulin-like growth factor IB, somatomedin-C

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

Citations for Recombinant Human IGF-I/IGF-1, Animal-Free Protein (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IGF-I/IGF-1, Animal-Free Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

R Sharma et al. (2021), Epithelial phenotype restoring drugs suppress macular degeneration phenotypes in an iPSC model Nature Communications, 12(1):7293.
PMID: 34911940
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay

Q Zheng et al. (2020), Cytoplasmic m1A reader YTHDF3 inhibits trophoblast invasion by downregulation of m1A-methylated IGF1R Cell Discov, 6(0):12.
PMID: 32194978

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