Product Specifications for Recombinant Human IFN-alpha/beta R1 Protein, CF
Mouse myeloma cell line, NS0-derived human IFN-alpha/beta R1 protein Gly26-Lys436
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
70-90 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human IFN-alpha / beta R1 is present at 1 μg/mL, it binds to Recombinant Human IFN‑ alpha / beta R2 Fc Chimera (Catalog # 4015-AB) in the presence of recombinant human IFN-alpha 2. The concentration of Recombinant Human IFN-alpha / beta R2 Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-1.5 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-alpha/beta R1
Interferon‑alpha/beta receptor 1 (IFN‑ alpha / beta R1), also known as IFNAR1, is a 100‑130 kDa member of the class II cytokine receptor family of proteins. These proteins form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). IFN‑ alpha / beta R1, in association with IFN‑ alpha / beta R2, is required for propagating anti‑microbial signal transduction triggered by the type 1 interferons such as IFN‑ alpha and IFN‑ beta (2, 3). Mature human IFN‑ alpha / beta R1 consists of a 409 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (4). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. Within the ECD, human IFN‑ alpha / beta R1 shares 47% and 50% aa identity with mouse and rat IFN‑ alpha / beta R1, respectively. Alternative splicing generates two additional isoforms that lack the transmembrane segment and either all or a portion of the cytoplasmic domain. IFN‑ alpha / beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN‑ alpha / beta R2. Ligands preferentially associate with IFN‑ alpha / beta R2, and this complex subsequently forms a stable ternary assembly with IFN‑ alpha / beta R1 (5‑7). IFN‑ alpha / beta R1 also associates with IFN‑ gamma R2 even in the absence of IFN‑ gamma stimulation (3). IFN‑ alpha / beta R1 activation depends on tyrosine phoshorylation as well as palmitoylation of its cytoplasmic domain (8, 9). Rapid down‑regulation of the receptor is accomplished by ligand‑dependent or ‑independent pathways (e.g. VEGF R signaling, TLR signaling, or cellular stress) which induce its serine phosphorylation, ubiquitination, and degradation (10‑13).
Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284.
Takaoka, A. et al. (2000) Science 288:2357.
Uze, G. et al. (1990) Cell 60:225.
Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
Kalie, E. et al. (2008) J. Biol. Chem. 283:32925.
Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
Claudinon, J. et al. (2009) J. Biol. Chem. 284:24328.
Zheng, H. et al. (2011) Blood 118:4003.
Qian, J. et al. (2011) PLoS Pathogens 7:e1002065.
Bhattacharya, S. et al. (2010) J. Biol. Chem. 285:2318.
Bhattacharya, S. et al. (2011) J. Biol. Chem. 286:22069.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics