Source	Human embryonic kidney cell, HEK293-derived IFN-alpha B2/IFNA8 protein Cys24-Glu189
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Cys24
Predicted Molecular Mass	19 kDa
SDS-PAGE	23-27 kDa, under reducing conditions.
Activity	Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 1.20-12.0 pg/mL.

Recombinant Human IFN-alpha B2/IFNA8 Protein, CF Scientific Data Examples

	Recombinant Human IFN-alpha B2/IFNA8 Protein Bioactivity. Recombinant Human IFN-alpha B2/IFNA8 Protein (Catalog # 11018-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 1.20-12.0 pg/mL
	Recombinant Human IFN-alpha B2/IFNA8 Protein SDS-PAGE. 2 μg/lane of Recombinant Human IFN-alpha B2/IFNA8 Protein (Catalog # 11018-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 23-27 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11018-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha B2/IFNA8

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2-4). Structurally, type I IFNs belong to the class of five helical-bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds (5). The extracellular domain (ECD) of mature human IFNA8, also known as IFN‑alpha B2, shares 60% aa sequence identity with mouse homolog. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha-subunit) and IFNAR2 (beta -subunit) (6, 7). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA8 is the most potent IFNA, judging by both antiviral and antiproliferative activities (8). The reason for this higher potency is unstructured tail region of IFNA8 that gains structure during complex formation and might explain the differences in activity between IFNAs (8). IFNA8 has been shown to exhibit antiproliferative and cytotoxic effects on renal cancer cell (RCC) lines and chronic myelogenous leukemia (CML)-derived cell lines (9, 10).

Item	Value
References	Pestka, S. et al. (1987) Annu. Rev. Biochem. 56:727. Goeddel, D.V. et al. (1980) Nature 287:411. Matsumiya, T. et al. (2007) J. Immunol. 179:4542. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139. Wittling, M.C. et al. (2021) Front Immunol. 11:605673. van Pesch, V. et al. (2004) J. Virol. 78:8219. James, C.M. et al. (2007) Vaccine. 25(10):1856. Slutzki, M. et al. (2006) J Mol Biol. 360:1019. Yanai, Y. et al. (2002) Cancer Letter 185:173. Yanai, Y. et al. (2001) J. Interferon Cytokine Res 21:1129.
Long Name	Interferon alpha 8
Entrez Gene IDs	3445 (Human)
Alternate Names	IFN-alpha 8, IFN-alpha B2, IFN-alpha-8, IFN-alphaB, IFNA8, IFNalpha B2, Interferon alpha-B, Interferon alpha-B2, LeIF B, interferon alpha type 201, interferon alpha-8, interferon alpha-B', interferon, alpha 8

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Recombinant Human IFN-alpha B2/IFNA8 Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

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Background: IFN-alpha B2/IFNA8

Citations for Recombinant Human IFN-alpha B2/IFNA8 Protein, CF

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