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Recombinant Human IFN-alpha 10/IFNA10 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11016-IF

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11016-IF-010
11016-IF-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IFN-alpha 10/IFNA10 protein
Cys24-Asp189

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-22 kDa, under reducing conditions.

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 1.00-30.0 pg/mL.

Scientific Data Images for Recombinant Human IFN-alpha 10/IFNA10 Protein, CF

Recombinant Human IFN-alpha 10/IFNA10 Protein Bioactivity.

Recombinant Human IFN-alpha 10/IFNA10 Protein (Catalog # 11016-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 1.00-30.0 pg/mL

Recombinant Human IFN-alpha 10/IFNA10 Protein SDS-PAGE.

2 μg/lane of Recombinant Human IFN-alpha 10/IFNA10 Protein (Catalog # 11016-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.

Formulation, Preparation and Storage

11016-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 10/IFNA10

Interferons (IFN) are a family of cytokines with potent anti‑viral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2 - 4). Structurally, type I IFNs belong to the class of five helical‑bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds (5). Mature human IFNA10 shares 61% aa sequence identity with mouse IFNA7. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (6, 7). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA10 has been shown to be a strong inducer of IFN-stimulated genes and anti‑viral protection (8). Additionally, IFNA10 exhibits weak anti‑viral effects against SARS-CoV-2 (9).

References

  1. Pestka, S. et al. (1987) Annu. Rev. Biochem. 56:727.
  2. Goeddel, D.V. et al. (1980) Nature 287:411. 
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  5. Wittling, M.C. et al. (2021) Front Immunol. 11:605673.
  6. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  7. James, C.M. et al. (2007) Vaccine. 25(10):1856.
  8. Moll, H.P. et al. (2011) Cytokine. 53:52.
  9. Schuhenn, J. et al. (2022) PNAS https://doi.org/10.1073/pnas.2111600119.

Long Name

Interferon alpha 10

Alternate Names

IFN-alpha C, IFNA10, IFNalpha C

Entrez Gene IDs

3446 (Human)

Gene Symbol

IFNA10

UniProt

Additional IFN-alpha 10/IFNA10 Products

Product Documents for Recombinant Human IFN-alpha 10/IFNA10 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IFN-alpha 10/IFNA10 Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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