Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF

Name: Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF
Brand: R&D Systems
SKU: BT169

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BT169

Product Datasheet / COA / SDS

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q9Y6W8
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF

Source

Mouse myeloma cell line, NS0-derived human ICOS protein

Human ICOS (Glu21-Phe141) Accession # Q9Y6W8	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Biotinylated via sugars

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu21

Predicted Molecular Mass

40 kDa (unlabeled)

SDS-PAGE

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED₅₀ of 1-6 ng/mL.

Scientific Data Examples for Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF

	Recombinant Human ICOS Fc Chimera Biotinylated Protein Binding Activity When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant ICOS Fc Chimera (Catalog # BT169) binds with an ED₅₀ of 1-6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT169

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 400 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICOS

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on T_h primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

Item	Value
References	Aicher, A. et al. (2000) J. Immunol. 164:4689. Coyle, A.J. et al. (2000) Immunity 13:95. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1. Hutloff, A. et al. (1999) Nature 397:263. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name	Inducible Co-stimulator
Entrez Gene IDs	29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names	AILIM, AILIMCD278, Activation-inducible lymphocyte immunomediatory molecule, CD278, CD278 antigen, CRP-1, CVID1, ICOS, MGC39850, inducible T-cell co-stimulator, inducible T-cell costimulator, inducible costimulator

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