Product Specifications for Recombinant Human HTRA2/Omi Protein, CF
E. coli-derived human HTRA2/Omi protein Ala134-Glu458, with a C-terminal 6-His tag
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
N-terminal sequence Analysis
Predicted Molecular Mass
38 kDa, reducing conditions
Measured by its ability to cleave beta -casein. >95% cleavage of beta -casein, as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
HtrA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HtrA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HtrA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (Catalog # 786-XB) inhibition of Caspase-7 (Catalog # 823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC50 values for this effect are typically between 0.2 and 1.5 μM. HtrA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site. The specificity of the protease is yet to be defined and no endogenous substrates are known to date.
Suzuki, Y. et al. (2001) Mol. Cell. 8:613.
van Loo, G. et al. (2002) Cell Death & Diff. 9:20.
Hedge, R. et al. (2001) J. Biol. Chem. 277:432.
Verhagen, A. et al. (2001) J. Biol. Chem. 277:445.
Martins, L. et al. (2002) J. Biol. Chem. 277:439.
Silke, J., and A. Verhagen (2002) Cell Death & Diff. 9:362.
Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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