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Recombinant Human HTRA2/Omi Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1458-HT

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1458-HT-100

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human HTRA2/Omi protein
Ala134-Glu458, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

N-terminal Sequence Analysis

Ala134

Predicted Molecular Mass

36 kDa

SDS-PAGE

38 kDa, reducing conditions

Activity

Measured by its ability to cleave beta-casein.
>95% cleavage of beta-casein, as measured under the described conditions.

Formulation, Preparation and Storage

1458-HT
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: HTRA2/Omi

HtrA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HtrA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HtrA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (Catalog # http://www.rndsystems.com/product_results.aspx?k=786-XB">786-XB) inhibition of Caspase-7 (Catalog # http://www.rndsystems.com/product_results.aspx?k=823-C7">823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC50 values for this effect are typically between 0.2 and 1.5 μM. HtrA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site. The specificity of the protease is yet to be defined and no endogenous substrates are known to date.

References

  1. Suzuki, Y. et al. (2001) Mol. Cell. 8:613.
  2. van Loo, G. et al. (2002) Cell Death & Diff. 9:20.
  3. Hedge, R. et al. (2001) J. Biol. Chem. 277:432.
  4. Verhagen, A. et al. (2001) J. Biol. Chem. 277:445.
  5. Martins, L. et al. (2002) J. Biol. Chem. 277:439.
  6. Silke, J., and A. Verhagen (2002) Cell Death & Diff. 9:362.
  7. Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.

Long Name

High Temperature Requirement Protein-2

Alternate Names

Omi, PRSS25

Entrez Gene IDs

27429 (Human); 64704 (Mouse)

Gene Symbol

HTRA2

UniProt

Additional HTRA2/Omi Products

Product Documents for Recombinant Human HTRA2/Omi Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human HTRA2/Omi Protein, CF

For research use only

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