Recombinant Human HSP70/HSPA1A Protein, CF

Name: Recombinant Human HSP70/HSPA1A Protein, CF
Brand: R&D Systems
SKU: AP-100
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AP-100

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Source:	E. coli
Accession #:	AAD21816
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human HSP70/HSPA1A Protein, CF

Source	E. coli-derived human HSP70/HSPA1A protein
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass	70 kDa
Activity	HSP70/HSPA1A is a molecular chaperone that assists in the folding of nascent polypeptides and the refolding of denatured proteins, but can also promote their degradation in conjunction with specific E3 ligases, such as CHIP, if either of these processes proceeds inefficiently. Reaction conditions will need to be optimized for each specific application. We recommend an initial HSP70/HSPA1A concentration of 2-3 μM for in vitro use. IMPORTANT: HSP40/DNAJB1 (Catalog # AP-110), or another suitable co-chaperone, is required for HSP70/HSPA1A activity and should be used at a concentration that is equimolar to HSP70/HSPA1A.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-100

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 8, 100 mM NaCl, 5 mM DTT
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

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Background: HSP70/HSPA1A

Heat Shock 70kDa Protein (HSP70), also known as Heat Shock 70kDa Protein 1A (HSPA1A), is a 641 amino acid (aa) member of the HSP70 family of molecular chaperones with a predicted molecular weight of 70 kDa. Human HSP70/HSPA1A shares 95% and 97% aa sequence identity with the mouse and rat orthologs, respectively. It has an N-terminal nucleotide-binding domain, which contains ATPase activity, and a C-terminal substrate-binding domain (1). HSP70/HSPA1A promotes the proper folding of nascent polypeptides and assists in the refolding of denatured proteins (2). However, if either of these processes proceeds too slowly or fails, HSP70/HSPA1A can interact with the HSP40 co-chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein (3,4). HSP70/HSPA1A can be regulated post-translationally via multiple mechanisms, including phosphorylation, ubiquitination, and methylation (5-8). For example, unmethylated HSP70/HSPA1A localizes to the cytoplasm, but following methylation on Lys561 it is found only in the nucleus (8). Pathologically, HSP70/HSPA1A has been implicated in the promotion of multiple cancer types (8-10). Conversely, it is thought to protect against several neurodegenerative diseases that are caused by the accumulation of misfolded proteins (11,12).

Item	Value
References	Qi, R. et al. (2013) Nat. Struct. Mol. Biol. [Epub ahead of print]. Kampinga, H.H. & E.A. Craig (2010) Nat. Rev. Mol. Cell Biol. 11:579. McDonough, H. & C. Patterson (2003) Cell Stress Chaperones 8:303. Donnelly, B.F. et al. (2013) J. Biol. Chem. 288:13124. Muller, P. et al. (2012) Oncogene [Epub ahead of print]. Liu, M. et al. (2008) J. Biol. Chem. 283:35783. Moore, D.J. et al. (2008) J. Neurochem. 105:1806. Cho, H.S. et al. (2012) Nat. Commun. 3:1072. Wu, F.H. et al. (2012) Cancer Lett. 317:157. Gaudio, E. et al. (2013) Blood 121:351. Miyata, Y. et al. (2011) Future Med. Chem. 3:1523. Wang, A.M. et al. (2013) Nat. Chem. Biol. 9:112.
Long Name	Heat Shock Protein 70
Entrez Gene IDs	3303 (Human); 15511 (Mouse); 24472 (Rat)
Alternate Names	FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, HSP70, HSP70-1, HSP70-1/HSP70-2, HSP70-1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1/2, dnaK-type molecular chaperone HSP70-1, heat shock 70 kDa protein 1A/1B, heat shock 70kD protein 1A, heat shock 70kDa protein 1A, heat shock-induced protein

Citations for Recombinant Human HSP70/HSPA1A Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human HSP70/HSPA1A Protein, CF include:

Species: Rat
Sample Types: In Vivo
Applications: Bioassay

K Kanno et al. (2020), Role of macrophage-mediated Toll-like receptor 4-interleukin-1R signaling in ectopic tongue pain associated with tooth pulp inflammation J Neuroinflammation, 17(1):312.
PMID: 33081813
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

Ohara et al. (2013), Toll-like receptor 4 signaling in trigeminal ganglion neurons contributes tongue-referred pain associated with tooth pulp inflammation. J Neuroinflammation, 10(0):139.
PMID: 24267924

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Customer Reviews for Recombinant Human HSP70/HSPA1A Protein, CF (2)

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Recombinant Human HSP70/HSPA1A Protein, CF

Name: Anonymous

Application: Enzymatic activity in vitro

Verified Customer | Posted 11/07/2018
Recombinant Human HSP70/HSPA1A Protein, CF

Name: Neda Ghousifam

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 08/17/2018

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Product Documents for Recombinant Human HSP70/HSPA1A Protein, CF

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Recombinant Human HSP70/HSPA1A Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human HSP70/HSPA1A Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: HSP70/HSPA1A

Citations for Recombinant Human HSP70/HSPA1A Protein, CF (2)

Customer Reviews for Recombinant Human HSP70/HSPA1A Protein, CF (2)

Recombinant Human HSP70/HSPA1A Protein, CF

Recombinant Human HSP70/HSPA1A Protein, CF

Product Documents for Recombinant Human HSP70/HSPA1A Protein, CF

Product Datasheets

COA

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