Recombinant Human HMGB1/HMG-1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11803-HM

R&D Systems, part of Bio-Techne
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11803-HM-050
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Key Product Details

Source

E. coli

Accession #

P09429.3

Conjugate

Unconjugated

Applications

Bioactivity

View all HMGB1/HMG-1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human HMGB1/HMG-1 protein
Met1-Asp215, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly2

Predicted Molecular Mass

26 kDa

SDS-PAGE

28-32 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human HMGB1/HMG‑1 His-tag binds to Recombinant Human RAGE Fc Chimera Protein (Catalog # 1145-RG) with an ED50 of 0.040-0.400 μg/mL.

Scientific Data Images for Recombinant Human HMGB1/HMG-1 His-tag Protein, CF

Recombinant Human HMGB1/HMG‑1 His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human HMGB1/HMG‑1 His-tag Protein (Catalog # 11803-HM) binds to Recombinant Human RAGE Fc Chimera Protein (1145-RG) with an ED50 of 0.040-0.400 μg/mL.

Recombinant Human HMGB1/HMG‑1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human HMGB1/HMG‑1 His-tag Protein (Catalog # 11803-HM) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 28-32 kDa.

Formulation, Preparation and Storage

11803-HM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HMGB1/HMG-1

High-mobility Group Box 1 protein (HMGB1), also known as HMG1 or Amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1-3). Human HMGB1 is expressed as a 25 kDa single chain protein containing two globular positively charged DNA-binding domains (HMG boxes A and B) and a negatively charged C-terminal region that contains only Asp and Glu residues (4, 5). Posttranslational modification of HMGB1, including acetylation, phosphorylation, and methylation, affects HMGB1 localization, receptor interactions, and bioactivity (3). An intramolecular disulfide bond between Cys23 and Cys45 as well as the presence of the unpaired Cys106 thiol are critical for HMGB1-induced pro-inflammatory TNF-alpha secretion (2, 6). Alternatively, fully reduced HMGB1 acts as a potent chemoattractant for neutrophils and monocytes (7). HMGB1 can be localized to the nucleus or cytoplasm and can also be secreted despite its lack of a signal peptide (2). HMGB1 binds DNA in a non-sequence specific manner and may act as a structural cofactor during gene transcription (8). Acetylation of HMGB1 results in its cytoplasmic localization and eventual secretion (9). HMGB1 can be secreted by multiple cell types, and it is also released upon cell necrosis, apoptosis, and pyroptosis (2, 3). HMGB1 is widely recognized as a multifunctional alarmin that stimulates inflammation upon sterile or infectious insult. Receptors for HMGB1 include TLR2, TLR4, TLR9, Syndecan-3, Siglec-10, Integrin alpha M beta 2, CXCR4, TIM-3, and RAGE (1, 2). It is implicated in the pathogenesis of a broad range of diseases including atherosclerosis, sepsis, cancer, neurodegenerative diseases, and autoimmunity (3, 10-13).

References

  1. Yanai, H. et al. (2012) Trends Immunol. 33:633.
  2. Yang, H. et al. (2013) J. Leukoc. Biol. 93:865.
  3. Harris, H.E. et al. (2012) Nat. Rev. Rheumatol. 8:195.
  4. Degryse, B. and M. de Virgilio (2003) FEBS Lett. 553:11.
  5. Wen, L. et al. (1989) Nucleic Acids Res. 17:1197.
  6. Yang, H. et al. (2021) Mol. Med. 27:58.
  7. Venereau, E. et al. (2012) J. Exp. Med. 209:1519.
  8. Bianchi, M.E. and A. Agresti (2005) Curr. Opin. Genet. Dev. 15:496.
  9. Bonaldi, T. et al. (2003) EMBO J. 22:5551.
  10. Diener, K.R. et al. (2013) Immunol. Cell Biol. 91:443.
  11. Fang, P. et al. (2012) Mol. Neurobiol. 45:499.
  12. de Souza, A.W. et al. (2012) Autoimmun. Rev. 11:909.
  13. Bae, J.S. (2012) Arch. Pharm. Res. 35:1511.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Entrez Gene IDs

3146 (Human)

Gene Symbol

HMGB1

UniProt

P09429.3

Additional HMGB1/HMG-1 Products

Product Documents for Recombinant Human HMGB1/HMG-1 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human HMGB1/HMG-1 His-tag Protein, CF

For research use only

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