Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF

Catalog # SP-600 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

O60885.2

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human BRD4 protein
Glu49 - Glu460 with N-terminal 8-His and (DYKDDDDK) tags

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

52 kDa

Activity

This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders. Reaction conditions will need to be optimized for each specific application.

Scientific Data Images for Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF

Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein SDS-PAGE

Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein SDS-PAGE

1 μg per lane of Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460 (Catalog # SP-600) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.

Formulation, Preparation and Storage

SP-600
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: BRD4

Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory.  BRD4 has two N-terminal bromodomains and one NET (N-terminal Extra Terminal) domain.  BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II.  BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.  This recombinant protein contains an N-terminal portion of BRD4 including both bromodomains. This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders.

References

  1. Devaiah, B.N. et al. (2012) Proc. Natl. Acad. Sci. 109:6927.
  2. Gadd, M.S. et al. (2017) Nat. Chem. Biol. 13:514.
  3. Wang, R. et al. (2012) J. Biol. Chem. 287:10738.
  4. Zhang, W. et al. (1994) J. Biol. Chem. 287:43137.

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Entrez Gene IDs

23476 (Human); 57261 (Mouse)

Gene Symbol

BRD4

UniProt

O60885.2

Product Documents for Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF

For research use only

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