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Recombinant Human HGF Activator Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1514-SE

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1514-SE-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Pro form

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human HGF Activator protein
Gln36-Ser655, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln36

Predicted Molecular Mass

68 kDa

SDS-PAGE

105 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >15 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1514-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: HGF Activator

Hepatocyte Growth Factor Activator (HGFA) is a serine endopeptidase that cleaves at the peptide bond between Arg494 and Val495 of single-chain human HGF precursor, generating the active heterodimer (1). HGFA is produced and secreted by the liver and normally circulates in the blood as an inactive zymogen (2, 3). The zymogen has a weak affinity for heparin but acquires a strong affinity for heparin upon activation that is linked to blood coagulation. This property may ensure the local action of this enzyme at the site of tissue injury (3). Human HGFA precursor (655 amino acid residues) contains several predicted domains including a signal peptide (residues 1‑30), a propeptide (residues 31-372), and a mature and active form (residues 373-655) that is further processed into a short chain (residues 373‑407) and a long chain (residues 408-655). The short chain and the long chain (catalytic domain) may form a disulfide bond linked dimer. HGFA can be activated by thrombin (Catalog # http://www.rndsystems.com/product_results.aspx?k=1473-SE">1473-SE)  or thermolysin (Catalog # http://www.rndsystems.com/product_results.aspx?k=3097-ZN">3097-ZN)  (4). The active protease can be inhibited by HGFA inhibitors (HAIs). Two HAIs, HAI-1 and HAI-2, are known in mouse and human. HAI-1 is not only an inhibitor, but also a specific acceptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (5).

References

  1. Kitamura, N. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. Eds.) p. 1712, Academic Press, San Diego.
  2. Miyazawa, K. et al. (1993) J. Biol. Chem. 268:10024.
  3. Miyazawa, K. et al. (1996) J. Biol. Chem. 271:3615.
  4. Shimomura, T. et al. (1993) J. Biol. Chem. 268:22927.
  5. Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.

Long Name

Hepatocyte Growth Factor Activator

Alternate Names

HGFA, Hgfac

Entrez Gene IDs

3083 (Human); 54426 (Mouse)

Gene Symbol

HGFAC

UniProt

Additional HGF Activator Products

Product Documents for Recombinant Human HGF Activator Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human HGF Activator Protein, CF

For research use only

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