Source	Mouse myeloma cell line, NS0-derived human HAI-2A protein Met1-Lys197, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala28
Predicted Molecular Mass	21 kDa
SDS-PAGE	27 kDa and 32 kDa, reducing conditions
Activity	Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (Catalog # ES002). The IC₅₀ value is <4.6 nM, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1106-PI

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl₂.
Reconstitution	Reconstitute at 100 μg/mL in sterile, deionized water. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HAI-2A

Two alternatively spliced forms of HAI-2 have been found in human tissues (1). HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. Encoded by the SPINT2 gene, HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain (1‑5). Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trysin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa. In comparison, HAI-2A is a weaker inhibitor of factor VIIa-tissue factor, factors IXa, Xa, and XIIa. Recombinant HAI-2A prolonged the clotting time in an activated partial thromboplastin time assay.

Item	Value
References	Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096. Kawaguchi, T. et al. (1997) J. Biol. Chem. 272:27558. Marlor. C.W. et al. (1997) J. Biol. Chem. 272:12202. Muller-Pillasch, F. et al. (1998) Biochim. Biophys. Acta 1395:88. Delaria, K.A. et al. (1997) J. Biol. Chem. 272:12209.
Long Name	HGF Activator Inhibitor Type 2A
Entrez Gene IDs	10653 (Human)
Alternate Names	HAI-2A, HAI2A, Placental Bikunin, SPINT2

Citations for Recombinant Human HAI-2A Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human HAI-2A Protein, CF include:

Sample Types: Protein
Applications: Bioassay

R Szabo et al. (2018), Loss of HAI-2 in mice with decreased prostasin activity leads to an early-onset intestinal failure resembling congenital tufting enteropathy PLoS ONE, 13(4):e0194660.
PMID: 29617460
Species: Human
Sample Types: Whole Cells
Applications: Activation

Zoratti G et al. (2015), Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling. Nat Commun, 6(0):6776.
PMID: 25873032
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Szabo R et al. (2008), Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity. J. Biol. Chem., 283(43):29495-504.
PMID: 18713750

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Recombinant Human HAI-2A Protein, CF

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