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Recombinant Human HAI-2A Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1106-PI

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1106-PI-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human HAI-2A protein
Met1-Lys197, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala28

Predicted Molecular Mass

21 kDa

SDS-PAGE

27 kDa and 32 kDa, reducing conditions

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <4.6 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1106-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Reconstitution
Reconstitute at 100 μg/mL in sterile, deionized water.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2A

Two alternatively spliced forms of HAI-2 have been found in human tissues (1). HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. Encoded by the SPINT2 gene, HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain (1‑5). Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trysin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa. In comparison, HAI-2A is a weaker inhibitor of factor VIIa-tissue factor, factors IXa, Xa, and XIIa. Recombinant HAI-2A prolonged the clotting time in an activated partial thromboplastin time assay.

References

  1. Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
  2. Kawaguchi, T. et al. (1997) J. Biol. Chem. 272:27558.
  3. Marlor. C.W. et al. (1997) J. Biol. Chem. 272:12202.
  4. Muller-Pillasch, F. et al. (1998) Biochim. Biophys. Acta 1395:88.
  5. Delaria, K.A. et al. (1997) J. Biol. Chem. 272:12209.

Long Name

HGF Activator Inhibitor Type 2A

Alternate Names

HAI2A, Placental Bikunin, SPINT2

Entrez Gene IDs

10653 (Human)

Gene Symbol

SPINT2

UniProt

Additional HAI-2A Products

Product Documents for Recombinant Human HAI-2A Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human HAI-2A Protein, CF

For research use only

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Reconstitution Calculator

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