Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF

Name: Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF
Brand: R&D Systems
SKU: E-306

R&D Systems, Inc. a Bio-Techne Brand

Catalog # E-306

Citations (5)

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Key Product Details

Source:	Sf 9 (baculovirus)
Accession #:	P22314
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF

Source	Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein Contains an N-terminal GST (glutathione S-transferase) tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass	148 kDa
Activity	Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) concentration of 50-200 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-306

Formulation	X mg/ml (X µM) in 50 mM HEPES pH 7.5, 50 mM NaCl
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

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Background: Ubiquitin-activating Enzyme/UBE1

Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa. It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11). UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions. This protein has an N-terminal GST tag.

Item	Value
References	Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258. Nagai, Y. et al. (1995) J. Cell Sci. 108:2145. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206. Schulman, B.A. & J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319. Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543. Haas, A.L. & I.A. Rose (1982) J. Biol. Chem. 257:10329. Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115. Nouspikel, T. & P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188. Moundry, P. et al. (2012) Cell Cycle 11:1573. Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.
Entrez Gene IDs	7317 (Human); 22201 (Mouse); 314432 (Rat); 100009225 (Rabbit); 853670 (Yeast)
Alternate Names	A1S9T, A1S9T and BN75 temperature sensitivity complementing, A1ST, AMCX1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, UBA1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1A, UBE1, UBE1A1S9, UBE1X, Ubiquitin-activating Enzyme, Ubiquitin-activating enzyme E1, Ubiquitinactivating Enzyme, ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing), ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier-activating enzyme 1

Citations for Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF include:

Species: N/A
Sample Types: Plasmid
Applications: Ubiquitination

L Kiss et al. (2021), RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination Nature Communications, 12(1):1220.
PMID: 33619271
Species: Human
Sample Types: Protein
Applications: Enzyme Assay

(2016), Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation Sci Rep, 6(0):30955.
PMID: 27485036
Species: N/A
Sample Types: Protein
Applications: Bioassay

(2016), Lunapark Is a Component of a Ubiquitin Ligase Complex Localized to the Endoplasmic Reticulum Three-way Junctions J Biol Chem, 0(0).
PMID: 27387505
Sample Types: Recombinant Protein
Applications: Bioassay

Rodrigo-Brenni M et al. (2014), Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell, 55(2):227-37.
PMID: 24981174
Species: Human
Sample Types: Whole Cells

Pan Y et al. (2014), Smurf2 negatively modulates RIG-I-dependent antiviral response by targeting VISA/MAVS for ubiquitination and degradation. J Immunol, 192(10):4758-64.
PMID: 24729608

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