Recombinant Human Growth Hormone (GH) Protein

Name: Recombinant Human Growth Hormone (GH) Protein
Brand: R&D Systems
SKU: 1067-GH

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1067-GH

Citations (6)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Source:	E. coli
Accession #:	CAA23779.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Growth Hormone (GH) Protein

Product Specifications for Recombinant Human Growth Hormone (GH) Protein

Source	E. coli-derived human Growth Hormone protein Phe27-Phe217, with an N-terminal Met
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	22 kDa
Activity	Measured in a cell proliferation assay using Nb2‑11 rat lymphoma cells. Gout, P.W. et al. (1980) Cancer Res. 40:2433. The ED₅₀ for this effect is 0.025-0.1 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1067-GH

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1067-GH/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Growth Hormone

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin (3) and leptin (4). The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified (5).

Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types (1, 2). GH stimulates the liver and other tissues to produce IGF-1, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-1. GH, directly or indirectly via IGF-1, can act on B cells, T cells, NK cells, macrophages and neutrophils to exert immunomodulatory activities (6). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake and protein synthesis (1, 2, 6).

Item	Value
References	Goffin, V. et al. (1996) Endocrine Rev. 17:385. Le Roith, D. et al. (2001) Endocrine Rev., 22:53. Kojima, K. et al. (1999) Nature, 402:656. Tannenbaum, G. et al. (1998) Endocrinol. 139:3871. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
Entrez Gene IDs	2688 (Human)
Alternate Names	GH-N, GH-Nsomatotropin, GH1, GHB5, GHIGHD1B, GHN, GHNGrowth hormone, Growth Hormone, IGHD1A, IGHD2, Somatotropin, growth hormone 1Pituitary growth hormone, hGH-N

Citations for Recombinant Human Growth Hormone (GH) Protein (6)

Showing 1 - 6 of 6 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Growth Hormone (GH) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Growth hormone is permissive for neoplastic colon growth Proc Natl Acad Sci USA, 0(0).
PMID: 27226307
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lombardi S et al. (2014), Growth hormone is secreted by normal breast epithelium upon progesterone stimulation and increases proliferation of stem/progenitor cells. Stem Cell Reports, 2(6):780-93.
PMID: 24936466
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhao Y et al. (2014), Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-alpha, and IL-1beta. Am J Physiol Endocrinol Metab, 307(2):E186-98.
PMID: 24895283
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Suh H et al. (2013), Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators. J Neuroinflammation, 10(0):37.
PMID: 23497056
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Krejci P et al. (2009), Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes. Cell. Signal., 21(1):151-60.
PMID: 18950705
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Banziger-Tobler NE et al. (2008), Growth hormone promotes lymphangiogenesis. Am. J. Pathol., 173(2):586-97.
PMID: 18583315

Recombinant Human Growth Hormone (GH) Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Product Specifications for Recombinant Human Growth Hormone (GH) Protein

Formulation, Preparation and Storage

Carrier Free

1067-GH

Carrier

1067-GH/CF

Carrier Free

Reconstitution Calculator

Background: Growth Hormone

Citations for Recombinant Human Growth Hormone (GH) Protein (6)

Product Documents for Recombinant Human Growth Hormone (GH) Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected