Recombinant Human GOT1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11696-G1

R&D Systems, part of Bio-Techne
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human GOT1 His-tag Protein (11696-G1)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

P17174.3

Conjugate

Unconjugated

Applications

Enzyme Activity

View all GOT1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human GOT1 protein
Ala2-Gln413, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

47 kDa

SDS-PAGE

38-42 kDa under reducing conditions.

Activity

Measured by its ability to produce L-glutamate from L-aspartate in a coupled reaction.
The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human GOT1 His-tag Protein, CF

Recombinant Human GOT1 His-tag Protein Enzyme Activity.

Recombinant Human GOT1 His-tag Protein (Catalog # 11696-G1) is measured by its ability to produce L-glutamate from L-aspartate in a coupled reaction.

Recombinant Human GOT1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human GOT1 His-tag Protein (Catalog # 11696-G1) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-42 kDa under reducing conditions.

Formulation, Preparation and Storage

11696-G1
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: GOT1

Glutamate oxaloacetate transaminase 1 (GOT1), also known as cytoplasmic Aspartate aminotransferase (ATTC) belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family (1). GOT1 is broadly expressed across tissues, organs, and cells (2, 3). GOT1 has an isozyme, GOT2, with 48% similarity (4) that differs in cellular localization. While the GOT2 isozyme is the mitochondrial form, GOT1 is the cytosolic form. GOT1 is an active homodimer of monomers with a small domain that contains the N- and C-terminus and a large domain that contains the core structure and dimerization surface. Each monomer contains an active site and a pyridoxal 5'-phosphate (PLP) cofactor (5). GOT1 is involved in biosynthesis of L-glutamate from L-aspartate or L-cysteine and functions as a critical enzyme in the malate-aspartate shuttle where it plays a role in the regulation of metabolic activity in human cells and also in cancer cells where it is required to maintain enhanced glycolysis to support proliferation (1, 3). GOT1 is overexpressed in numerous cancers including pancreatic, colon, breast, prostate, and leukemina and correlated with poor prognosis (6-11) giving it value as a diagnostic marker (1,3).  In addition, GOT-1 expression has been reported as an indicator for liver dysfunction, cardiovascular disease, and diabetes (12). Given the broad implications of GOT1 involvement in disease and as a cancer metabolic target of interest, there is significant interest in GOT-1 as a therapeutic target (3, 8,13,14). 

References

  1. Peng, H. et al. (2024) Front. Oncol. 14:1519046. 
  2. Ladue, J.S. et al. (1954) Science. 120:497.
  3. Song, Z. et al. (2022) Eur. J. Pharmacol. 917:174754. 
  4. Doyle, J.M. et al. (1990) Biochem. J. 270:651. 
  5. Malashkevich, V.N. et al. (1995) J. Mol. Biol. 247:111. 
  6. Feld, F.M. et al. (2015) Oncotarget 6:4516.
  7. Yang, Y. (2016) Biochem. Biophys. Res. Commun. 473:1295.
  8. Hong, C. et al. (2017) Cancer Chemother. Pharmacol. 79:835.
  9. Cheng, Z. et al. (2020) Front. Oncol. 10:379.
  10. Singh, A.N. et al. (2020) Front. Oncol. 10:493.
  11. Siwo, G.H. et al. (2024) preprint. doi: 10.1101/2024.03.01.582939.
  12. Monami, M. et al. (2008) Metabolism 57:387.
  13. Holt, M.C. et al. (2018) Biochemistry 57:6604. 
  14. Wang, Q. et al. (2019) Bioorg. Chem. 93:103315.

Long Name

Glutamic-oxaloacetic transaminase 1

Alternate Names

AST1, ASTQTL1, SGOT

Entrez Gene IDs

2805 (Human); 14718 (Mouse); 24401 (Rat)

Gene Symbol

GOT1

UniProt

P17174.3

Additional GOT1 Products

Product Documents for Recombinant Human GOT1 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human GOT1 His-tag Protein, CF

For research use only

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