Recombinant Human GM2/GD2 Synthase/B4GALNT1 His-tag, CF

R&D Systems, part of Bio-Techne | Catalog # 11777-BG

R&D Systems, part of Bio-Techne
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11777-BG-020
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Key Product Details

Source

CHO

Accession #

Q00973.2

Conjugate

Unconjugated

Applications

Enzyme Activity

View all GM2/GD2 Synthase/ B4GALNT1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human GM2/GD2 Synthase/ B4GALNT1 protein
Ser26-Gln533, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

57 kDa

SDS-PAGE

61-68 kDa, under reducing conditions.

Activity

Measured by its ability to hydrolyze UDP-GalNAc. The specific activity is >18 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human GM2/GD2 Synthase/B4GALNT1 His-tag, CF

Recombinant Human GM2/GD2 Synthase/ B4GALNT1 His-tag Enzyme Activity Diagram.

Recombinant Human GM2/GD2 Synthase/ B4GALNT1 His-tag (Cat # 11777-BG) catalyzes the transfer of GalNAc onto ceramides (Cer) such as GM3 and GD3 by a beta-1,4 linkage, resulting in the formation of products such as GM2 and GD2.

Recombinant Human GM2/GD2 Synthase/ B4GALNT1 His-tag SDS-PAGE.

2 μg/lane of Recombinant Human GM2/GD2 Synthase/ B4GALNT1 His-tag (Catalog # 11777-BG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-68 kDa, under reducing conditions.

Formulation, Preparation and Storage

11777-BG
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: GM2/GD2 Synthase/ B4GALNT1

Beta-1,4 N-acetylgalactosaminyltransferase 1 (B4GALNT1), also known as GalNAc-T or GM2/GD2 synthase from the gene B4GALNT1, is a glycosylated, Mn-dependent type-II Golgi transmembrane homodimeric protein that belongs to the CAZy GT12 family of glycosyltransferases (GTs) (1-3). B4GALNT1 is a key enzyme of the ganglioside (sialic acid-containing glycosphingolipids) synthetic pathway, responsible for the synthesis of the complex gangliosides GM2 and GD2 from their respective precursors GM3 and GD3 (4); these products are the essential precursors of the major brain gangliosides. B4GALNT1 has a predicted domain structure consisting of a short 7 amino acid N-terminal cytoplasmic sequence followed by a transmembrane domain and a 56 kDa lumenal domain (3). Each monomer luminal domain of the B4GALNT1 homodimer possesses an N-terminal region that forms a non-catalytic domain with a section that wraps across the dimer and contribute loops and helices to both the upper and lower domains, while the C-terminal region forms much of the predicted catalytic domain including a conserved DxD motif required for UDP-binding and the manganese ion (3). In addition, there are hydrophobic residues present on two surface loops flanking the active site that insert into the lipid bilayer to allow the enzyme to process membrane-embedded substrates. The dimer is arranged in an antiparallel orientation with highly conserved disulfides including an inter-chain disulfide bond (3, 5). B4GALNT1 loss of function is caused by several mutations that disrupt the substrate binding site or dimerization site of the protein (3) resulting in a complex early-onset form of hereditary spastic paraplegia 26 (HSP26) (6). Overexpression or increased activity of B4GALNT1 is associated with several cancers and diseases including childhood neuroblastoma, hepatocellular carcinoma (HCC), Systemic Lupus Erythematosus (SLE) (3, 7-10). Upregulated catalytic products are present in GM2 gangliosidoses such as Tay-Sach's, Sandhoff, and AB variant, and can be antibody-mediated chemotherapeutic targets (11-13) or targeted for substrate reduction strategies (3, 14). Direct inhibition of B4GALNT1 may also represent an auxiliary therapeutic strategy along with immunotherapy in HCC where the enzyme plays a role in immunosuppression (15), as potential therapeutics in cancers where overexpression is driving pathogenesis, or where GM2 substrate reduction would be beneficial (3, 15).

References

  1. Jaskiewicz, E.et al. (1996) J. Biol. Chem. 271:26395.
  2. Abidi, I. et al. (2025) Molecules 30:3615. 
  3. Welland, J.W. et al. (2025) Nat. Commun. 16:5442
  4. Pohlentz, G. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7044.
  5. Li, J. et al. (2000) J. Biol. Chem. 275:41476.
  6. Alecu, J.E. et al. (2022) Am. J. Med. Genet. A. 188:2590.
  7. Schengrund, C.-L.(2020) Int. J. Mol.Sci. 21:5313.
  8. Yoshida, H. et al. (2020) Sci. Rep. 10:1199.
  9. Liu, W. et al. (2023) J. Gene Med. 25:e3552.
  10. Katsuyama, E. et al. (2024) Nat. Commun. 15:8304.
  11. Sait, S. and S. Modak (2017) Expert Rev. Anticancer Ther. 17:889.
  12. Nazha, B. et al. (2020) Front. Oncol. 10:1000.
  13. Chan, G. C.-F. and C.M Chan. (2022) Biomolecules 12:358.
  14. Mansouri, V. et al. (2023) Eur. J. Neurol. 30:2919.
  15. Wang, Z. et al. (2024) Mol. Biomed. 5:65.

Long Name

Beta-1,4-N-Acetyl-Galactosaminyltransferase 1

Alternate Names

GALGT, GALNACT, GD2 Synthase, GM2 Synthase, SPG26

Entrez Gene IDs

2583 (Human); 14421 (Mouse)

Gene Symbol

B4GALNT1

UniProt

Q00973.2

Additional GM2/GD2 Synthase/ B4GALNT1 Products

Product Documents for Recombinant Human GM2/GD2 Synthase/B4GALNT1 His-tag, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human GM2/GD2 Synthase/B4GALNT1 His-tag, CF

For research use only

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