Recombinant Human Glypican 3 Fc Chimera Protein, CFNew

Name: Recombinant Human Glypican 3 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 11078-GP

Catalog # 11078-GP

Product Datasheet / COA / SDS

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Key Product Details

Source:	HEK293
Accession #:	P51654.1
Structure / Form:	Disulfide-linked homodimer. Glypican 3 is subject to endoproteolytic processing by proprotein convertases (PC). By amino acid sequencing, Two subunits (Alpha and Beta) are present (Alpha inferred to start with Gln25 and Beta starting with Ser359). They are associated via disulfide bonds.
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Glypican 3 Fc Chimera Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Glypican 3 protein

Human Glypican 3 (Gln25-His559) Accession # P51654.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained. Gln25 inferred from enzymatic pyroglutamate treatment revealing Pro26 and Ser359.

Predicted Molecular Mass

87 kDa (monomer)

SDS-PAGE

230-330 kDa, under non-reducing conditions.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human FGF basic/FGF2/bFGF (Catalog # 233-FB/CF) is immobilized at 0.500 µg/mL (100 µL/well), Recombinant Human Glypican 3 Fc Chimera Protein binds with an ED₅₀ of 0.0120-0.120 µg/mL.

Recombinant Human Glypican 3 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human Glypican 3 Fc Chimera Protein Binding Activity. When Recombinant Human FGF basic/FGF2/bFGF (233-FB) is immobilized at 0.500 µg/mL (100 µL/well), Recombinant Human Glypican 3 Fc Chimera Protein (Catalog # 11078-GP) binds with an ED₅₀ of 0.0120-0.120 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11078-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glypican 3

Glypican 3 (GPC3), also known as OCI5 and MXR7, is a member of the heparan sulfate proteoglycan (HSPG) family (1). In mammals, six glypican family members have been identified, all sharing a structurally common extracellular domain (ECD) with a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C‑terminal GPI attachment site. The ECD of GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N‑terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C‑terminal beta subunit containing two HS glycan chains (1-3). Within ECD, human GPC3 shares 96% amino acid sequence identity with both mouse and rat GPC3. Several isoforms of GPC3 due to alternative splicing have been reported (1). GPC3 is widely expressed on the membrane of various embryonic cells, but not on those in adult liver, and is involved in the regulation of growth and development of the body (4). GPC3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5). GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (6). Loss-of-function mutations in GPC3 are associated with Simpson-Golabi-Behmel (SGB) syndrome, a condition characterized by tissue overgrowth (dysmorphogenesis) (7).

Item	Value
References	Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333. Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275. Shimizu, Y. et al. (2019) Front Oncol. 9:248. Gonzales, A.D. et al. (1998) J. Cell Biol. 141:1407. Xue, Y. et al. (2018) Am J Pathol. 188(6):1469. Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774. Pilia, G. et al. (1996) Nature Genet.12:241.
Entrez Gene IDs	2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)
Alternate Names	DGSX, GPC3, GTR2-2, Glypican 3, Intestinal protein OCI-5, MXR7, OCI-5, OCI5, SGB, SGBS, SGBS1SDYS, glypican proteoglycan 3, glypican-3, heparan sulphate proteoglycan, secreted glypican-3

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