Recombinant Human Glutaminase His-tag Protein, CF

Name: Recombinant Human Glutaminase His-tag Protein, CF
Brand: R&D Systems
SKU: 10115-GL

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10115-GL

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Source:	E. coli
Accession #:	O94925-1
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human Glutaminase His-tag Protein, CF

Product Specifications for Recombinant Human Glutaminase His-tag Protein, CF

Source	E. coli-derived human Glutaminase protein Ser17-Leu669 with N-terminal Met and 6-His tag
Purity	>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	73 kDa
SDS-PAGE	59-74 kDa, under reducing conditions
Activity	Measured by its ability to hydrolyze glutamine to glutamate. The specific activity is >12000 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human Glutaminase His-tag Protein, CF

	Recombinant Human Glutaminase His-tag Protein Enzyme Activity Recombinant Human Glutaminase His-tag (Catalog # 10115-GL) is measured by its ability to hydrolyze glutamine to glutamate. The activity (orange) is higher than the competitor's Glutaminase (green).
	Recombinant Human Glutaminase His-tag Protein SDS-PAGE 1 μg/lane of Recombinant Human Glutaminase His-tag (Catalog # 10115-GL) and 1 μg/lane of competitor Human Glutaminase was resolved with 4-20% SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10115-GL

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP, Glycerol and CHAPS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

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Background: Glutaminase

Glutaminase (GLS) catalyzes the synthesis of glutamate from glutamine. There are two mammalian isozymes of glutaminase: kidney-type encoded by the GLS gene, and liver-type encoded by the GLS2 gene. The two isozymes are known to have different localization, functionality, and immunological and molecular characteristics (1, 2). The GLS gene expresses two splice variants with a conserved catalytic domain (3): kidney-type glutaminase (KGA) and a C-terminal truncated variant known as glutaminase C. Both splice variants form active tetramers and are activated by phosphate (4). The kidney glutaminase isoform, GLS, catalyzes the first reaction in the primary pathway for renal catabolism of glutamine to glutamate. It contains a mitochondrial signaling peptide, a nuclear receptor box, a catalytic glutaminase domain, three ankyrin repeats and a KEN box (5). GLS promotes respiration and ATP generation in the mitochondria by providing an alternative input to the citric acid cycle. GLS is upregulated in response to oncogenes (5-7) in several cancers (8, 9). Cancer cells with altered metabolism have been shown to depend on GLS activity and upregulated glutamine consumption (10) for growth in acute myeloid leukemina (11), breast cancer (12), colorectal cancer (13), kidney cancer (14), lung cancer (15), melanoma (16) and pancreatic cancer (17). Based on its role in cancer, GLS is a pharmaceutical target for cancer therapy (10, 12, 13, 18).

Item	Value
References	Kovacevic, Z. et al. (1983) Physiol. Rev. 63:547. Marquez, J. et al. (2006) Neurochem. Int. 48:465. Ramachandran, S. et al. (2016) Oncotarget. 7:57943. Huang, Q. et al. (2018) J. Biol. Chem. 293:3535. Thangavelu, K. et al. (2012) Proc. Natl. Acad. Sci. 109:7705. Wise, D. et al. (2008) Proc. Natl. Adac. Sci. 105:18782. Gao, P. et al. (2009) Nature. 458:762. Cline, M. S. et al. (2013) Sci. Rep. 3:2652. Cancer Genome Atlas Research Network, et al. (2013) Nat. Genet. 45:1113. Katt, W. P. et al. (2017) Future Med. Chem. 9:223. Willems, L. et al. (2013) Blood 122:3521. Gross, M. I. et al. (2014) Mol. Cancer Ther. 13:890. Huang, F. et al. (2014) Int. J. Clin. Exp. Pathol. 7:1093. Shroff, E. H. et al. (2015) Proc. Natl. Acad. Sci. 112:6539. van den Heuvel, A. P. et al. (2012) Cancer Biol. Ther. 13:1185. Hernandez-Davies, J. E. et al. (2015) J. Trans. Med. 13:1. Son, J. et al. (2013) Nature. 496:101. Zimmermann, S.C. et al. (2018) J. Med. Chem. [Epub ahead of print].
Long Name	Glutaminase Kidney Isoform, Mitochondrial
Entrez Gene IDs	2744 (Human); 14660 (Mouse); 24398 (Rat)
Alternate Names	AAD20, EC 3.5.1.2, GLS, GLS1DKFZp686O15119, Glutaminase, K-Glutaminase, KIAA0838FLJ10358, L-Glutamine Amidohydrolase, glutaminase C, glutaminase kidney isoform, mitochondrial, glutaminase, phosphate-activated

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Recombinant Human Glutaminase His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Glutaminase His-tag Protein, CF

Scientific Data Examples for Recombinant Human Glutaminase His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Glutaminase

Citations for Recombinant Human Glutaminase His-tag Protein, CF

Product Documents for Recombinant Human Glutaminase His-tag Protein, CF

Product Datasheets

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