Recombinant Human GIPR Fc Chimera Protein, CF

Name: Recombinant Human GIPR Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 11088-GI

Catalog # 11088-GI | R&D Systems, Inc. a Bio-Techne Brand

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	P48546.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human GIPR Fc Chimera Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human GIPR Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human GIPR protein

Human GIPR (Gly26-Gln138) Accession # P48546.1	IEGRMD	Human IgG₁ Fc (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly26

Predicted Molecular Mass

40 kDa

SDS-PAGE

40 kDa

Activity

Measured by its binding ability in a functional ELISA. When Biotinylated Human GIP Peptide is captured on a Streptavidin Coated Plate (Catalog # CP004), it binds to Recombinant Human GIPR Fc Chimera. The ED₅₀ for this binding is 20.0-100 ng/mL.

Scientific Data Examples for Recombinant Human GIPR Fc Chimera Protein, CF

	Recombinant Human GIPR Fc Chimera Protein Binding Activity. When Biotinylated Human GIP Peptide is captured on a Streptavidin Coated Plate (CP004), it binds to Recombinant Human GIPR Fc Chimera Protein (Catalog # 11088-GI). The ED₅₀ for this binding is 20.0‑100 ng/mL.
	Recombinant Human GIPR Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Human GIPR Fc Chimera Protein (Catalog # 11088-GI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-59 kDa and 90-120 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11088-GI

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: GIPR

Human Gastric Inhibitory Polypeptide Receptor (GIPR) is a transmembrane G protein coupled receptor that is mainly found in beta-cells within the pancreas (1). GIPR has 117 aa extracellular domain on its N-terminus, a central region consisting of seven transmembrane domains and a 68 aa C-terminal cytoplasmic domain that is responsible for intracellular transduction with the G-Protein (2). GIPR has three known isoforms produced by alternative splicing. The extracellular domain of human GIPR shows 76.3% and 81.2% amino acid identity with mouse and rat homolog, respectively. When originally discovered, GIPR was thought to have a role of inhibiting the secretion of gastrin and gastric acid (1). However, it was subsequently discovered that GIPR's main function was to stimulate the release of insulin in the presence of elevated blood glucose levels (1). GIPR has been found to bind to glucagon-like peptide-1 (GIP) and cascades downstream to release insulin (1). GIP and its receptor (GIPR) are of high pharmacological interest, since expression of GIPR are found in different organs and systems, especially in identification and design of new molecules for the treatment of diabetes mellitus and obesity (3-6). GIPR has also been shown to have an indirect relation with bone health and density. Mouse overexpressing GIP and GIPR had increased levels of osteoblasts and an overall decrease in age-related bone loss, while mice with GIPR knockout showed a decrease in overall bone mass and a higher level of compromised bone mass (7). Targeted Radionuclide Therapy (TRT) against GIPR positive cancer cells showed a significant increase of cell cycle arrest, specifically at the G2 and M phase, along with extensive DNA damage (8).

Item	Value
References	YAMADA, Y. et al. (1995). Genomics 29:773. Parthier, C. et al. (2007). Proc.Natl. Acad. Sci. U.S.A. 104:13942. Sherman, S.K. et at. Surgery. (2013) 154(6):. doi:10.1016/j.surg.2013.04.052. Yagub, T. et al. (2010) Molecular Pharmacology. 77(4):547. Volz, A. et al. (1995) Febs Letters. 373(1):23. Kieffer, T.J. et al. (2003) Trends Pharmacol Sci 24:110. Torekov, S.S. et al. (2014) J Clin Endocrinol Metab. 99:E729. Shi, X, et al. (2021) Bioconjug Chem. 32:1763.
Long Name	Gastric Inhibitory Polypeptide Receptor
Entrez Gene IDs	2696 (Human); 381853 (Mouse); 25024 (Rat)
Alternate Names	GIP-R, GIPR, Glucose-dependent insulinotropic polypeptide receptor, MGC126722, PGQTL2, gastric inhibitory polypeptide receptor

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Recombinant Human GIPR Fc Chimera Protein, CF

Catalog # 11088-GI | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human GIPR Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human GIPR Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: GIPR

Citations for Recombinant Human GIPR Fc Chimera Protein, CF

Product Documents for Recombinant Human GIPR Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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