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Recombinant Human GCNT2 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10847-GT

Glucosaminyl (N-acetyl) Transferase 2
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10847-GT-020

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Glucosaminyl (N-acetyl) Transferase 2/GCNT2 protein
Asn26-Phe400, with a C-terminal 6-His

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn26

Predicted Molecular Mass

43.8 kDa

SDS-PAGE

53-67 kDa, under reducing conditions.

Activity

Measured by its ability to transfer GlcNAc from UDP-GlcNAc to Cy5-labeled Extended G2.

0.25 μg of Recombinant Human GCNT2 will convert >50% of its substrate to product, as measured under the described conditions.

Scientific Data Images for Recombinant Human GCNT2 His-tag Protein, CF

Recombinant Human GCNT2 His-tag Protein/GCNT2 Enzyme Activity Diagram.

GCNT2 (Catalog # 10847-GT) recognizes polylactosamine structure and can tolerate core-6 fucose and its Cy5 derivatives.

Recombinant Human GCNT2 His-tag Protein, CF SDS-PAGE

1 μg/lane of Recombinant Human GCNT2 His-tag (Catalog # 10847-GT) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 53-63 kDa.

Recombinant Human GCNT2 His-tag Protein, CF Fluorescent Gel Mobility Shift

Lane 1 contained substrate glycan extended G2 (GL303). In the presence of rhGCNT2, the glycan was modified, and a mobility shift was observed.

Formulation, Preparation and Storage

10847-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Glucosaminyl (N-acetyl) Transferase 2/GCNT2

N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase (GCNT2) is a key branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. It is responsible for the formation of the blood group I antigens during embryonic development and is closely associated with the development and maturation of erythroid cells (1, 2, 3). GCNT2 is a Golgi resident type II membrane protein with a typical domain structure of glycosyltransferases. GCNT2 has been found to be downregulated in melanomas, which leads to the loss of N-linked I-branched glycans and the synthesis of poly-N-acetyllactosamine (i-linear) glycans (4). In addition, GCNT2 has been found to play a role in breast cancer and lung cancer and knockdown of GCNT2 expression decreased cell migration and invasion in vitro (5). The activity of recombinant GCNT2 is demonstrated in an electrophoretic gel mobility shift assay using a fluorophore-labeled glycan as the substrate (6).

References

  1. Marti, F.A. et al. (1995) Glycobiology 5:417.
  2. Bierhuizen, M.F.A. et al. (1993) Genes Dev. 7:468.
  3. Inaba, N. et al. (2003) blood 101:2870.
  4. Sweeney, Jenna Geddes et al. (2018) Nature communications 9:3368.
  5. Haijun, Zhang et al. (2011) Cancer Res. 71:4846.
  6. Wu, Z.L. et al (2020) Glycobiology 30:970.

Long Name

Glucosaminyl (N-Acetyl) Transferase 2, I-Branching Enzyme (I Blood Group)

Alternate Names

CCAT, CTRCT13, GCNT2C, NACGT1, NAGCT1, ULG3

Entrez Gene IDs

2651 (Human); 14538 (Mouse)

Gene Symbol

GCNT2

UniProt

Additional Glucosaminyl (N-acetyl) Transferase 2/GCNT2 Products

Product Documents for Recombinant Human GCNT2 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human GCNT2 His-tag Protein, CF

For research use only