Recombinant Human Galectin-8 Protein

Name: Recombinant Human Galectin-8 Protein
Brand: R&D Systems
SKU: 1305-GA
Rating: 4 (1 reviews)

Catalog # 1305-GA | R&D Systems, Inc. a Bio-Techne Brand

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Citations (4)

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Key Product Details

Source:	E. coli
Accession #:	O00214
Applications:	Bioactivity

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Technical Data
Citations (4) / Reviews (1)

Product Specifications for Recombinant Human Galectin-8 Protein

Source	E. coli-derived human Galectin-8 protein Met1-Trp317
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met1
Predicted Molecular Mass	36 kDa
SDS-PAGE	36 kDa
Activity	Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED₅₀ for this effect is ≤10 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1305-GA

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG 8000 and Trehalose with BSA as a carrier protein.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1305-GA/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG 8000 and Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-8

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N‑acetyl‑lactosamine‑containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (‑1, ‑2, ‑5, ‑7, ‑10, ‑11, ‑13, ‑14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem‑repeat Galectins (‑4, ‑6, ‑8, ‑9, ‑12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat‑type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM‑integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7).Human and mouse Galectin-8 share approximately 80% amino acid homology (4).

Item	Value
References	Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741. Hughes, R.C. (2002) Biochimie 83:667. R&D Systems’ Cytokine Bulletin, Summer, 2002. Bidon, N. et al. (2001) Gene 274:253. Hadari, Y. et al. (1995) J. Biol. Chem. 270:3447. Levy, Y. et al. (2001) J. Biol. Chem. 276:31285.
Entrez Gene IDs	3964 (Human); 56048 (Mouse)
Alternate Names	GAL8, Gal-8, Galectin-8, Galectin8, LGALS8, PCTA-1Po66 carbohydrate-binding protein, PCTA1, Po66 carbohydrate binding protein, Po66-CBP, Prostate carcinoma tumor antigen 1, galectin 8, galectin-8g, lectin, galactoside-binding, soluble, 8

Citations for Recombinant Human Galectin-8 Protein (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Galectin-8 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Negi et al. (2021-10-20), Computational repurposing of therapeutic small molecules from cancer to pulmonary hypertension Science Advances, 2021-17(43):eabh3794.
PMID: 34669463
Species: Human
Sample Types: Basement Membrane Extract
Applications: Bioassay

Ž Boji?-Trbo et al. (2019-02-14), Human trophoblast requires galectin-3 for cell migration and invasion Sci Rep, 2019-09(1):2136.
PMID: 30765738
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lhuillier C et al. (2015-05-06), Impact of Exogenous Galectin-9 on Human T Cells: CONTRIBUTION OF THE T CELL RECEPTOR COMPLEX TO ANTIGEN-INDEPENDENT ACTIVATION BUT NOT TO APOPTOSIS INDUCTION. J Biol Chem, 2015-0290(27):16797-811.
PMID: 25947381
Species: Human

Applications: Binding Assay

Cueni LN et al. (2009-03-04), Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions. Exp. Cell Res., 2009-0315(10):1715-23.
PMID: 19268462

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Recombinant Human Galectin-8 Protein

Name: Anonymous

Verified Customer | Posted 10/26/2015

The protein is in an oligomeric form, therefore a lot of boiling is required before loading on western gel as a control. The R&D antibody was used to probe for this protein.

Recombinant Human Galectin-8 Protein

Catalog # 1305-GA | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human Galectin-8 Protein

Formulation, Preparation and Storage

Carrier Free

1305-GA

Carrier

1305-GA/CF

Carrier Free

Reconstitution Calculator

Background: Galectin-8

Citations for Recombinant Human Galectin-8 Protein (4)

Customer Reviews for Recombinant Human Galectin-8 Protein (1)

Customer Images

Recombinant Human Galectin-8 Protein

Product Documents for Recombinant Human Galectin-8 Protein

Product Datasheets

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SDS

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