Recombinant Human Galectin-3 (Human Cell-expressed), CF

Name: Recombinant Human Galectin-3 (Human Cell-expressed), CF
Brand: R&D Systems
SKU: 8259-GA
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8259-GA

Citations (2)

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Key Product Details

Source:	HEK293
Accession #:	P17931
Applications:	Bioactivity

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Product Specifications for Recombinant Human Galectin-3 (Human Cell-expressed), CF

Source	Human embryonic kidney cell, HEK293-derived human Galectin-3 protein Ala2-Ile250
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained: Ala2 predicted.
Predicted Molecular Mass	25 kDa
SDS-PAGE	26-31 kDa, reducing conditions
Activity	Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. The ED₅₀ for this effect id 0.6-3 μg/mL. Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED₅₀ for this effect is 3-10 μg/mL.

Scientific Data Examples for Recombinant Human Galectin-3 (Human Cell-expressed), CF

	Recombinant Human Galectin-3 (Human Cell-expressed) Bioactivity Recombinant Human Galectin-3 (Catalog # 8259-GA) supports the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. The ED₅₀ for this effect is 0.6-3 μg/mL, while competitor's protein shows very weak activity.
	Recombinant Human Galectin-3 (Human Cell-expressed) SDS-PAGE 1 μg/lane of Recombinant Human Galectin-3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 29.9 kDa and 29.8 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8259-GA

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-3

Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain (1, 2). Mature human Galectin-3 shares 78% and 79% amino acid (aa) sequence identity with mouse and rat Galectin-3, respectively. Human Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the crosslinking of cell surface oligosacchraides as well as integrin‑dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).

Item	Value
References	Robertson, M.W. et al. (1990) Biochemistry 29:8093. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679. Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181. Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172. Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305. Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616. Sano, H. et al. (2003) J. Clin. Invest. 112:389. Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841. Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264. Wen, Y. et al. (2006) J. Cell. Biochem. 98:115. Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177. Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160. Kohatsu, L. et al. (2006) J. Immunol. 177:4718. Nieminen, J. et al. (2008) J. Immunol. 180:2466. Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288. Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123. Sherwi, N. et al. (2012) Future Cardiol. 8:885. Shin, T. (2013) Acta Histochem. 115:407.
Entrez Gene IDs	3958 (Human); 16854 (Mouse)
Alternate Names	AGE-R3, CBP 35,35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBPCBP35, GALIG, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin-3, Galectin3, IgE-binding protein, L-31, L29, L31, LGALS2, LGALS3, Laminin-binding protein, Lectin L-29, MAC2GAL3, Mac-2, Mac-2 antigen, galactin-3, lectin, galactoside-binding, soluble, 3

Citations for Recombinant Human Galectin-3 (Human Cell-expressed), CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Galectin-3 (Human Cell-expressed), CF include:

Species: Human
Sample Types: Hippocampal Cell Lysates
Applications: IP

CC Tao et al. (2020), Galectin-3 promotes A&beta oligomerization and A&beta toxicity in a mouse model of Alzheimer's disease Cell Death Differ., 27(1):192-209.
PMID: 31127200
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MP Mathew et al. (2018), Distinctï¿½cargo-specific response landscapes underpin the complex and nuanced role of galectin-glycan interactions in clathrin-independent endocytosis J. Biol. Chem., 0(0).
PMID: 29581232

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Application: Immunoassay Standard

Verified Customer | Posted 09/30/2020

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Product Documents for Recombinant Human Galectin-3 (Human Cell-expressed), CF

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Recombinant Human Galectin-3 (Human Cell-expressed), CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human Galectin-3 (Human Cell-expressed), CF

Scientific Data Examples for Recombinant Human Galectin-3 (Human Cell-expressed), CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Galectin-3

Citations for Recombinant Human Galectin-3 (Human Cell-expressed), CF (2)

Customer Reviews for Recombinant Human Galectin-3 (Human Cell-expressed), CF (1)

Recombinant Human Galectin-3 (Human Cell-expressed), CF

Product Documents for Recombinant Human Galectin-3 (Human Cell-expressed), CF

Product Datasheets

COA

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