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Recombinant Human Frizzled-5 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1617-FZ

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1617-FZ-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Frizzled-5 protein
Human Frizzled-5
(Ala27-Pro167)
Accession # AAC50385
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

42.5 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated Wnt-5a in a functional ELISA with an estimated Kd <1.5 nM.

Formulation, Preparation and Storage

1617-FZ
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-5

Frizzled-5 is an approximately 62 kDa 7-TM glycoprotein that is one of at least ten Frizzled family Wnt receptors (1, 2). Wnt engagement of Frizzled, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co‑receptors, stabilizes beta-catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzleds can also signal through non-canonical pathways independently of LRPs (1). Mature human Frizzled-5 consists of a 212 amino acid (aa) extracellular domain (ECD), a 283 aa 7-TM region, and a 64 aa cytoplasmic domain with a PDZ binding motif (2, 3). The ECD includes a cysteine-rich region (CRD) that binds Wnts and is highly conserved among Frizzled proteins (1, 2, 4). Within the N-terminal ECD, human Frizzled-5 shares 95% aa sequence identity with mouse and rat Frizzled-5. Frizzled-5 is expressed in embryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung, and kidney), in the adult retina, colon, and pancreatic islets, some cancer cell lines, human embryonic stem cells, and in some monocyte and lymphocyte populations (2-8). Frizzled-5 functions as a receptor for Wnt-5a, Wnt-9b, Wnt-10b, Wnt-2b, and Wnt-7a (3, 5, 9, 10). It plays a role in the maintenance of yolk sac and placental vasculature (ishikawa) and in the regression of vitreous vasculature during eye development (5, 6, 11). It mediates the synaptogenic effect of Wnt-7a, contributes to the development of neuronal polarity, and is required for neuronal survival in the thalamus (3, 12, 13). In macrophages and monocytes, Frizzled-5 signaling induces the production of inflammatory cytokines (7, 8).

References

  1. Schulte, G. (2015) Eur. J. Pharmacol. 763:191.
  2. Saitoh T. et al. (2001) Int. J. Oncol. 19:105.
  3. Liu, C. et al. (2008) J. Neurosci. 28:5641.
  4. Katoh, Y. and M. Katoh (2007) Int. J. Oncol. 30:751.
  5. Ishikawa, T. et al. (2001) Development 128:25.
  6. Burns, C.J. et al. (2008) Dev. Dyn. 237:1614.
  7. Periera, C. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:504.
  8. Blumenthal, A. et al. (2006) Blood 108:965.
  9. He, X. et al. (1997) Science 275:1652.
  10. Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.
  11. Zhang, J. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5561.
  12. Sahores, M. et al. (2010) Development 137:2215.
  13. Slater, P.G. et al. (2013) PLoS One 8:e78892.

Alternate Names

Frizzled5, Frz-5, FZ-5, FZD5

Entrez Gene IDs

7855 (Human); 14367 (Mouse)

Gene Symbol

FZD5

UniProt

Additional Frizzled-5 Products

Product Documents for Recombinant Human Frizzled-5 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Frizzled-5 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human Frizzled-5 Fc Chimera Protein, CF (5)

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