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Recombinant Human FPRP/PTGFRN Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10433-FP

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10433-FP-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human FPRP/PTGFRN protein
Human FPRP/PTGFRN
(Arg22-Lys830)
Accession # Q9P2B2.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22

Predicted Molecular Mass

117 kDa

SDS-PAGE

120-140 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human FPRP/PTGFRN Fc Chimera (Catalog # 10433-FP) is immobilized at 5 µg/mL (100 µL/well), Biotinylated Recombinant Human CD9 Fc Chimera binds with an ED50 of 3-18 μg/mL.

Scientific Data Images for Recombinant Human FPRP/PTGFRN Fc Chimera Protein, CF

Recombinant Human FPRP/PTGFRN Fc Chimera Protein SDS-PAGE

Recombinant Human FPRP/PTGFRN Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human FPRP/PTGFRN Fc Chimera Protein (Catalog # 10433-FP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-140 kDa and 240-280 kDa, respectively.

Formulation, Preparation and Storage

10433-FP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP/PTGFRN

Prostoglandin F2 receptor negative regulator (PTGFRN), also known as CD9P-1, EWI-F, and CD315, is an integral type I transmembrane glycoprotein expressed on subsets of cells in ovary, uterus, lung, and heart (1-4). PTGFRN is a member of the Glu-Trp-Ile (EWI) subfamily within the Ig superfamily of molecules. The extracellular domain (ECD) of mature human PTGFRN contains six Ig-like C2-type domains and an EWI motif that acts as binding sites for actin-linking ezrin-radixin-moesin (ERM) proteins (1). The ECD of human PTGFRN shares 90% and 89% amino acid identity with mouse and rat PTGFRN, respectively. PTGFRN forms complexes with tetraspanins CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton and regulating cell motility and polarity (1, 2). Increased expression of CD9 and CD81 inhibits the HEK/PTGFRN cell motility on collagen-I (3). In addition, PTGFRN expression positively correlates with the metastatic status of hLT and the upregulation of PTGFRN expression could be a mechanism involved in the loss of CD9 in solid tumors, such as in lung cancer (4)

References

  1. Sala-Valdes, M. et al. (2006) J Biol Chem. 281:19665.
  2. Stipp, C. S. et al. (2001) J Biol Chem. 276:4853.
  3. Chambrion, C. et al. (2010) PLoS One. 5:11219.
  4. Guilmain, W. et al. (2011) Br. J Cancer. 104:496.

Long Name

Prostaglandin F2-alpha Receptor Regulatory Protein

Alternate Names

CD315, CD9P-1, PTGFRN

Entrez Gene IDs

5738 (Human); 19221 (Mouse); 29602 (Rat)

Gene Symbol

PTGFRN

UniProt

Additional FPRP/PTGFRN Products

Product Documents for Recombinant Human FPRP/PTGFRN Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human FPRP/PTGFRN Fc Chimera Protein, CF

For research use only

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