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Recombinant Human FLRT3 Protein, CF

Catalog # 2795-FL | R&D Systems, Inc. a Bio-Techne Brand
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2795-FL-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human FLRT3 protein
Lys29-Pro528, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys29

Predicted Molecular Mass

57.3 kDa     

SDS-PAGE

70-90 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells.
Recombinant Human FLRT3 immobilized at 5 μg/mL, 100 μL/well, will mediate >50% Neuro‑2A cell adhesion.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

2795-FL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLRT3

FLRT3 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85-95 kDa type I transmembrane (TM) human FLRT3 is synthesized as a 649 amino acid (aa) precursor with a 28 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa TM segment and a 100 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT3 ECD shares 96%, 96%, 97%, 97%, 98% and 81% aa sequence identity with mouse, rat, canine, bovine, equine and Xenopus FLRT3 ECD, respectively, and 61% and 48% aa identity to human FLRT2 and FLRT3 ECDs, respectively. The fibronectin domain is responsible for binding to FGF receptors, and is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization in areas of cell contact and homotypic cell-cell association (4). This may be through direct interaction with other FLRT molecules, or alternatively, by regulating internalization of adhesion molecules such as cadherins (4, 5). Developmentally, FLRT3 is located in somitic regions on dermatomyotomal muscle precursors and myotomal cells before their migration to the myotome and syndetome, respectively (2). FLRT3 is also expressed at the midbrain/hindbrain boundary and in the apical ectodermal ridge where it may influence FGF signaling (2). Genetic deletion in mouse embryos results in defective headfold fusion and endoderm migration (6). Postnatally, FLRT3 mRNA is widely expressed (1). It is up-regulated and promotes neurite outgrowth following experimental peripheral nerve injury in rats (7, 8).

 

References

  1. Lacy, S. E. et al. (1999) Genomics 62:417.
  2. Haines, B. P. et al. (2006) Dev. Biol. 297:14.
  3. Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.
  4. Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
  5. Ogata, S. et al. (2007) Genes Dev. 21:1817.
  6. Maretto, S. et al. (2008) Dev. Biol. 318:184.
  7. Tsuji, L. et al. (2004) Biochem. Biophys. Res. Commun. 313:1086.
  8. Robinson, M. et al. (2004) Mol. Cell. Neurosci. 27:202.

Long Name

Fibronectin Leucine Rich Transmembrane Protein 3

Alternate Names

fibronectin leucine rich transmembrane protein 3, Fibronectin-like domain-containing leucine-rich transmembrane protein 3, HH21, KIAA1469, leucine-rich repeat transmembrane protein FLRT3

Entrez Gene IDs

23767 (Human); 71436 (Mouse)

Gene Symbol

FLRT3

UniProt

Product Documents for Recombinant Human FLRT3 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human FLRT3 Protein, CF

For research use only

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