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Recombinant Human FGFR3 (IIIb) Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1264-FR

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1264-FR-01M
1264-FR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human FGF R3 protein
Human FGF R3 (IIIb)
(Glu23 - Gly377)
(Ala361 del)
Accession # NP_001156685
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu23

Predicted Molecular Mass

65 kDa (monomer)

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells.
The ED50 for this effect is 0.03-0.12 µg/mL.

Formulation, Preparation and Storage

1264-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR3

Fibroblast growth factors (FGFs) comprise a family of at least 23 structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1 - 3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1 - 3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

References

  1. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  2. Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.

Long Name

Fibroblast Growth Factor Receptor 3

Alternate Names

CD333, CEK, FGF R3, JTK4

Entrez Gene IDs

2261 (Human); 14184 (Mouse)

Gene Symbol

FGFR3

UniProt

Additional FGFR3 Products

Product Documents for Recombinant Human FGFR3 (IIIb) Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human FGFR3 (IIIb) Fc Chimera Protein, CF

For research use only

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    Application: Immunoassay Standard
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