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Recombinant Human FGFR2 alpha (IIIc) His-tag Protein, CF

Catalog # 11119-FR | R&D Systems, Inc. a Bio-Techne Brand
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11119-FR-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human FGFR2 alpha protein
Arg22-Glu377, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22

Predicted Molecular Mass

40 kDa

SDS-PAGE

58-72 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
In a Human FGF acidic/FGF1 antibody (Catalog # AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (Catalog # 232-FA), Human FGFR2 alpha (IIIc) His-tag Protein binds with an ED50 of 0.400-2.40 µg/mL.

Scientific Data Images for Recombinant Human FGFR2 alpha (IIIc) His-tag Protein, CF

Recombinant Human FGFR2 alpha (IIIc) His-tag Protein Bioactivity.

In a Human FGF acidic/FGF1 antibody (AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Human FGFR2 alpha (IIIc) His-tag Protein (Catalog # 11119-FR) binds with an ED50 of 0.400‑2.40 µg/mL.

Recombinant Human FGFR2 alpha (IIIc) His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human FGFR2 alpha (IIIc) His-tag Protein (Catalog # 11119-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-72 kDa.

Formulation, Preparation and Storage

11119-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2 alpha

Fibroblast growth factor receptor 2 (FGFR2) belongs to a family of type I transmembrane tyrosine kinases which mediate the biological functions of FGFs that are involved in a multitude of physiological and pathological cellular processes (1). The FGFR family is comprised of 4 structurally conserved members (FGFR1-4) all possessing an extracellular domain (ECD) with three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and cytoplasmic split tyrosine-kinase domain (1, 2). The ECD of mature, full-length FGFR2 shares 95% amino acid sequence identity with mouse FGFR2. Alternative splicing generates multiple forms of FGFR1-3, each with unique signaling characteristics (1-3). For FGFR2, alternative splicing of the ECD, specifically the IgIII domain, results in IIIb, or IIIc isoforms (4). The FGFR splice variants also exhibit distinct and varying binding affinities for different FGF ligands (2, 4). Specifically, FGFR2A (IIIc) binds most FGF ligands but not the FGF10 subfamily, while FGFR2A (IIIc) binds only members of the FGF10 subfamily (5). FGFRs mediate the FGF signaling cascade which regulate developmental processes including cellular proliferation, differentiation, and migration, morphogenesis, and patterning (6). FGFRs transduce the signals through three dominant pathways including RAS/MAPK, PI3k/AKT, and PLC gamma (7). While FGFR2 is widely expressed in many adult human tissues, isoform expression is tissue specific, with IIIb predominantly expressed in epithelial cells, while IIIc is expressed in mesenchymal cells (5). FGFR2 signaling is critical for embryonic development, tissue repair, and regulation of osteoblast function and bone growth (8). Mutations in FGFR2 or misregulation of FGFR2 mediated signaling is found in multiple skeletal dysplasias, with FGFR2A (IIIc) specifically upregulated in several cancers including prostate, breast and pancreatic and is proposed as a novel therapeutic target for colorectal carcinomas (6, 9).

References

  1. Ornitz, D.M. and Itoh, N. (2015) Wiley Interdiscip Rev Dev Biol. 4:215.
  2. Zhang, X. et al. (2006) J Biol Chem. 281:15694.
  3. Ferguson, H.R. et al. (2021) Signaling. Cells 10:1201.
  4. Holzmann, K. et al. (2012) J Nucleic Acids. 2012:950508.
  5. Wagner, E.J. et al. (2003) RNA 9:1552.
  6. Xie, Y. et al. (2020) Sig Transduct Target Ther 5:181.
  7. Mossahebi-Mohammadi, M. et al. (2020) Front Cell Dev Biol. 18:79.
  8. Teven, C.M. et al. (2014) Genes Dis. 1:199.
  9. Matsuda, Y. et al. (2012) Mol Cancer Ther. 11:2010.

Long Name

Fibroblast Growth Factor Receptor 2 alpha

Alternate Names

FGF R2a

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Gene Symbol

FGFR2

UniProt

Product Documents for Recombinant Human FGFR2 alpha (IIIc) His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human FGFR2 alpha (IIIc) His-tag Protein, CF

For research use only

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