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Recombinant Human FGF basic/FGF2 Protein, CF

Bio-Techne includes R&D Systems | Catalog # BT-FGFB

Catalog #
Availability
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BT-FGFB-01M
BT-FGFB-020
BT-FGFB-050
BT-FGFB-100
BT-FGFB-250
BT-FGFB-500

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human FGF basic/FGF2/bFGF protein
Ala135-Ser288

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala135 & Ala136

Predicted Molecular Mass

16 kDa

SDS-PAGE

18 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120; Watson, J.B. et al. (1994) J. Clin. Invest. 94:261.
The ED50 for this effect is 0.100-1.00 ng/mL.

Scientific Data Images for Recombinant Human FGF basic/FGF2 Protein, CF

Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human FGF basic/FGF2/bFGF.

Equivalent bioactivity of GMP (BT-FGFB-GMP), Animal-Free (BT-FGFB-AFL) and RUO (Catalog # BT-FGFB) grades of Recombinant Human FGF basic/FGF2/bFGF as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cell line (orange, green, red, respectively).

Recombinant Human FGF basic/FGF2/bFGF Protein Bioactivity.

Recombinant Human FGF basic/FGF2/bFGF (Catalog # BT-FGFB) stimulates proliferation of the NR6R-3T3 mouse fibroblast cell line. The ED50 for this effect is 0.100-1.00 ng/mL.

Recombinant Human FGF basic/FGF2/bFGF Protein SDS-PAGE.

2 μg/lane of Recombinant Human FGF basic/FGF2/bFGF Protein (Catalog # BT-FGFB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18 kDa.

Formulation, Preparation and Storage

BT-FGFB
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose.
Reconstitution Reconstitute the 20 μg size at 100 μg/mL in sterile deionized water. Reconstitute all other sizes at 500 μg/mL in sterile deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic (also known as FGF2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta-trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4 - 6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

References

  1. Sorenson, V. et al. (2006) BioEssays 28:504.
  2. Kardami, E. et al. (2004) Cardiovasc. Res. 63:458.
  3. Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115.
  4. Abraham, J.A. et al. (1986) EMBO J. 5:2523.
  5. Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836.
  6. Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505.
  7. Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254.
  8. Arese, M. et al. (1999) Mol. Biol. Cell 10:1429.
  9. Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249.
  10. Nickel, W. (2005) Traffic 6:607.
  11. SwissProt # P09038.
  12. Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839.
  13. Bailly, K. et al. (2000) FASEB J. 14:333.
  14. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Gene Symbol

FGF2

Additional FGF basic/FGF2/bFGF Products

Product Documents for Recombinant Human FGF basic/FGF2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human FGF basic/FGF2 Protein, CF

For research use only

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