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Recombinant Human FCRL2/FcRH2 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2048-FC

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2048-FC-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human FCRL2/FcRH2 protein
Leu20-Asp395, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

41.9 kDa

SDS-PAGE

60-68 kDa, reducing conditions

Activity

Measured by its ability to bind human IgG with an estimated KD <80 nM.

Formulation, Preparation and Storage

2048-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL2/FcRH2

Fc receptor-like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3 - 5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor-like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27+ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B-CLL) patients (10). FCRL2 upregulation is associated with mutation of the immunoglobulin heavy chain variable (IGHV) and less aggressive forms of B-CLL (9, 11).

References

  1. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  2. Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
  3. Miller, I. et al. (2002) Blood 99:2662.
  4. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
  5. Xu, M.J. et al. (2001) Biochem. Biophys. Res. Commun. 280:768.
  6. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  7. Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
  8. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  9. Huttmann, A. et al. (2006) Leukemia 20:1774.
  10. Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
  11. Li, F.J. et al. (2008) Blood 112:179.

Long Name

Fc Receptor-like 2

Alternate Names

CD307b, FcRH2, IFGP4, IRTA4, SPAP1

Entrez Gene IDs

79368 (Human)

Gene Symbol

FCRL2

UniProt

Additional FCRL2/FcRH2 Products

Product Documents for Recombinant Human FCRL2/FcRH2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human FCRL2/FcRH2 Protein, CF

For research use only

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