Product Specifications for Recombinant Human FCRL2/FcRH2 Protein, CF
Mouse myeloma cell line, NS0-derived human FCRL2/FcRH2 protein Leu20-Asp395, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
60-68 kDa, reducing conditions
Measured by its ability to bind human IgG with an estimated KD <80 nM.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Fc receptor-like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3 - 5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor-like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27+ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B-CLL) patients (10). FCRL2 upregulation is associated with mutation of the immunoglobulin heavy chain variable (IGHV) and less aggressive forms of B-CLL (9, 11).
Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
Miller, I. et al. (2002) Blood 99:2662.
Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
Xu, M.J. et al. (2001) Biochem. Biophys. Res. Commun. 280:768.
Hatzivassiliou, G. et al. (2001) Immunity 14:277.
Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
Huttmann, A. et al. (2006) Leukemia 20:1774.
Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
Li, F.J. et al. (2008) Blood 112:179.
Fc Receptor-like 2
Entrez Gene IDs
CD307b, CD307b antigen, FCRH2SPAP1B, FCRL2, Fc receptor homolog 2, Fc receptor-like 2, FcR-like protein 2, FcRH2, IFGP family protein 4, IFGP4, IRTA4, IRTA4SPAP1C, Immunoglobulin receptor translocation-associated protein 4, SH2 domain containing phosphatase anchor protein 1, SH2 domain-containing phosphatase anchor protein 1Fc receptor-like protein 2, SPAP1, SPAP1A, SPAP1immune receptor translocation-associated protein 4, immunoglobulin superfamily Fc receptor, gp42
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