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Recombinant Human Fc gamma RIIA/CD32a (R167) Protein Best Seller

Bio-Techne includes R&D Systems | Catalog # 1330-CD

His-tag
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Carrier Free
1330-CD-01M/CF
1330-CD-050/CF

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With Carrier
1330-CD-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Fc gamma RIIA/CD32a protein
Ala36-Ile218, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala36

Predicted Molecular Mass

22 kDa

SDS-PAGE

26-36 kDa, under reducing conditions.

Activity

Measured by its ability to bind human IgG with an estimated Kd <80 nM.

Scientific Data Images for Recombinant Human Fc gamma RIIA/CD32a (R167) Protein

Recombinant Human Fc gamma RIIA/CD32a (R167) Protein Binding Activity

Recombinant Human Fc gamma RIIA/CD32a (R167) Protein Binding Activity

Recombinant Human Fc gamma RIIA/CD32a (R167) (Catalog # 1330-CD) binds human IgG with an estimated KD<80 nM.
Recombinant Human Fc gamma RIIA/CD32a (R167) Protein SDS-PAGE

Recombinant Human Fc gamma RIIA/CD32a (R167) Protein SDS-PAGE

1 μg/lane of Recombinant Human Fc gamma RIIA/CD32a (R167) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 26-36 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1330-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1330-CD/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIA/CD32a

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1-3). The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).

Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified (1-3). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) (4, 5). Two allelic variants (R167 and H167) of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The H167 allele has been found to have a protective effect against lupus nephritis.

References

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Chi, M. et al. (2002) J. Immunol. 168:1413.
  5. Zuniga, R. et al. (2003) Arthritis Rheum. 48:460.

Long Name

Fc gamma Receptor II A

Alternate Names

CD32a, FCGR2A, FcgRIIA, FCRIIA

Entrez Gene IDs

2212 (Human); 14130 (Mouse); 116591 (Rat); 102143568 (Cynomolgus Monkey)

Gene Symbol

FCGR2A

UniProt

Additional Fc gamma RIIA/CD32a Products

Product Documents for Recombinant Human Fc gamma RIIA/CD32a (R167) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Fc gamma RIIA/CD32a (R167) Protein

For research use only

Citations for Recombinant Human Fc gamma RIIA/CD32a (R167) Protein (14)

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FAQs for Recombinant Human Fc gamma RIIA/CD32a (R167) Protein

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  • Q: Is the H131 or R131 allele encoded in Recombinant Human Fc gamma RIIA/CD32a (R167) Protein (Catalog # 1330-CD)?

    A: Catalog # 1330-CD protein is endcoded by the R131 allele. The protein sequence listed on our website (Ala36-Ile218, with a C-terminal 10-His tag, Accession# AAA35827) references R167. We have numbered this mutation at  residue 167, which takes into account the whole molecule including the signal peptide. If you look at just the mature sequence beginning at residue 36, then the mutation falls on residue 131.

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