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Recombinant Human ERMAP Fc Chimera Protein, CF

Catalog # 10645-ER | R&D Systems, Inc. a Bio-Techne Brand
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10645-ER-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ERMAP protein
Human ERMAP
(His30-Ala155)
Accession # Q96PL5.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His30 & Gly32

Predicted Molecular Mass

40 kDa

SDS-PAGE

40-55 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
ED50 for this effect is 0.75-7.5 μg/mL.

Scientific Data Images for Recombinant Human ERMAP Fc Chimera Protein, CF

Recombinant Human ERMAP Fc Chimera Protein Bioactivity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 0.75-7.5 μg/mL.

Recombinant Human ERMAP Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human ERMAP Fc Chimera (Catalog # 10645-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-55 kDa and 80-110 kDa, respectively.

Formulation, Preparation and Storage

10645-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERMAP

Erythroid membrane-associated protein (ERMAP), also called Scianna blood group antigen, is a member of the butrophilin (BTN) and butrophilin-like (BTNL) family within the immunoglobulin superfamily. ERMAP consists of an extracellular domain (ECD) with one V-type Ig-like (IgV) fold, a transmembrane segment, and a cytoplasmic domain with a B30.2 motif (1).  Within the ECD, human ERMAP shares 33% and 31% amino acid sequence identity with mouse and rat ERMAP, respectively. Interestingly, human ERMAP contains a single IgV-like domain in the ECD, while mouse and rat ERMAP contain both an IgV-like and IgC-like domain. However, the absence of the IgC-like domain in human ERMAP does not seem to affect its functions (2, 3). ERMAP is an adhesion receptor molecule highly expressed in erythroid tissues, on the cell surface of resting and activated antigen-presenting cells (APCs) and in some tumor tissues (1, 4, 5). Fusion proteins (ERMAP-Ig) of both human and mouse ERMAP inhibit T cell functions in vitro and administration of the fusion protein ameliorates autoimmune diseases, including experimental autoimmune encephalomyelitis and type 1 diabetes, in mice (5). ERMAP acts as a novel inhibitory molecule for T cells and macrophages (5).

References

  1. Xu, H. et al. (2001) Genomics 76:2.
  2. Compte, E.W. et al. (2004) Eur. J. Immunol. 34:2089.
  3. Su, Y.Y. et al. (2001) Blood Cell Mol. Dis. 27:938
  4. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  5. Su, M. et al. (2020) Cell. Mol. Immunol. doi: 10.1038/s41423-020-0494-8.

Long Name

Erythroblast Membrane-associated Protein

Alternate Names

RD, SC

Entrez Gene IDs

114625 (Human); 27028 (Mouse); 298485 (Rat)

Gene Symbol

ERMAP

UniProt

Product Documents for Recombinant Human ERMAP Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ERMAP Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

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