Recombinant Human Epiregulin Protein

Name: Recombinant Human Epiregulin Protein
Brand: R&D Systems
SKU: 1195-EP

Catalog # 1195-EP | R&D Systems, Inc. a Bio-Techne Brand

Citations (8)

Product Datasheet / COA / SDS

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Key Product Details

Source:	E. coli
Accession #:	O14944
Applications:	Bioactivity

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Technical Data
Citations (8) / Reviews

Product Specifications for Recombinant Human Epiregulin Protein

Source	E. coli-derived human Epiregulin protein Val63-Leu108, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	5.4 kDa
SDS-PAGE	5.4 kDa
Activity	Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED₅₀ for this effect is 0.125-0.75 ng/mL.

Scientific Data Examples for Recombinant Human Epiregulin Protein

	Recombinant Human Epiregulin Protein Bioactivity Recombinant Human Epiregulin (Catalog # 1195-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The ED₅₀ is 0.125-0.75 ng/mL.
	Recombinant Human Epiregulin Protein SDS-PAGE 1 μg/lane of Recombinant Human Epiregulin was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 5 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1195-EP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1195-EP/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Epiregulin

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. All EGF family members are synthesized as transmembrane precursors and are converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The human epiregulin cDNA encodes a 169 amino acid (aa) residues transmembrane precursor with a 29 aa signal peptide, a 21 aa transmembrane domain and a 21 aa cytoplasmic domain. The putative soluble mature Epiregulin comprising the EGF-like domain (aa residues 64-104) is formed by proteolytic removal of the propeptide regions (2). There is 85% aa sequence homology between human and mouse epiregulins. Epiregulin is expressed primarily in the placenta and macrophages (3). High level expression has also been detected in various carcinomas. Epiregulin specifically binds EGFR (ErbB1) and ErbB4 but not ErbB2 and ErbB3. It activates the homodimers of both ErbB1 and ErbB4. In addition, epiregulin can also activate all possible heteromeric combinations of the four ErbB family members (4). Epiregulin stimulates the proliferation of fibroblasts, smooth muscle cells and hepatocytes. It has been shown to be an autocrine growth factor for epidermal keratinocytes as well as mesangial cells (5, 6). Epiregulin has also been shown to inhibit growth of several epithelial tumor cells. In addition, Epiregulin has been implicated in the implantation process during pregnancy (7).

Item	Value
References	Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495. Toyoda, H. et al. (1997) Biochem. J. 326:69. Komurasaki, T. et al. (1997) Oncogene 15:2841. Shelly, M. et al. (1998) J. Biol. Chem. 273:10496. Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748. Mishre, R. et al. (2002) Am. J. Physiol. Renal. Physiol. 283:F1151. Das, S.K. et al. (1997) Dev. Biol. 190:178.
Entrez Gene IDs	2069 (Human); 13874 (Mouse)
Alternate Names	ER, EREG, Epiregulin, proepiregulin

Citations for Recombinant Human Epiregulin Protein (8)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Epiregulin Protein include:

Species: Mouse
Sample Types: Transfected Whole Cells
Applications: Bioassay

Farahani et al. (2023-05-22), pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells eLife
PMID: 37212240
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

TT Lemmetyine et al. (2023-04-03), Fibroblast-derived EGF ligand Neuregulin-1 induces fetal-like reprogramming of the intestinal epithelium without supporting tumorigenic growth Disease Models Mechanisms, 2023-04-03;0(0).
PMID: 36912192
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

NJ Song et al. (2022-01-26), Epiregulin as an Alternative Ligand for Leptin Receptor Alleviates Glucose Intolerance without Change in Obesity Cells, 2022-011(3).
PMID: 35159237
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Liu et al. (2020-08-25), EREG-driven oncogenesis of Head and Neck Squamous Cell Carcinoma exhibits higher sensitivity to Erlotinib therapy Theranostics, 2020-010(23):10589-10605.
PMID: 32929368
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Iijima et al. (2017-03-06), Epiregulin-blocking antibody inhibits epiregulin-dependent EGFR signaling Biochem. Biophys. Res. Commun, 2017-00(0).
PMID: 28274874
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Blaha M et al. (2015-10-06), Gene expression analysis of pig cumulus-oocyte complexes stimulated in vitro with follicle stimulating hormone or epidermal growth factor-like peptides. Reprod Biol Endocrinol, 2015-113(0):113.
PMID: 26445099
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Auf G et al. (2013-12-13), High epiregulin expression in human U87 glioma cells relies on IRE1alpha and promotes autocrine growth through EGF receptor. BMC Cancer, 2013-113(0):597.
PMID: 24330607
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Armant DR et al. (2006-01-11), Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor. Development, 2006-0133(4):751-9.
PMID: 16407398

Recombinant Human Epiregulin Protein

Catalog # 1195-EP | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human Epiregulin Protein

Scientific Data Examples for Recombinant Human Epiregulin Protein

Formulation, Preparation and Storage

Carrier Free

1195-EP

Carrier

1195-EP/CF

Carrier Free

Reconstitution Calculator

Background: Epiregulin

Citations for Recombinant Human Epiregulin Protein (8)

Product Documents for Recombinant Human Epiregulin Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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