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Recombinant Human Epigen Protein

Bio-Techne includes R&D Systems | Catalog # 6629-EP

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Carrier Free
6629-EP-025/CF

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With Carrier
6629-EP-025

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human Epigen protein
Leu54-Ala104, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

6 kDa

SDS-PAGE

5.5 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.

The ED50 for this effect is 100-500 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 6629-EP
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 6629-EP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Epigen

Epigen (EPGN) is an epithelial mitogen that belongs to the EGF superfamily (1 ‑ 3). Human Epigen cDNA encodes a 154 amino acid (aa) residue type I transmembrane precursor glycoprotein with a 22 aa signal peptide, an 88 aa extracellular domain, a 21 aa transmembrane domain and a 23 aa cytoplasmic domain (2, 3). The extracellular domain (aa 23 ‑ 110) contains a pattern of six cysteines typical of EGF family proteins (1 ‑ 3). Potential splice variants of 133, 112, 103, 94 or 73 aa, lacking either N‑terminal sequence (aa 15 or 36 to 44), transmembrane and juxtamembrane sequence (aa 87 or 96 to 137), or sequences in both regions, have been described (4). In addition to potentially secreted splice variants, Epigen is among several EGF family proteins that can undergo metalloproteinase cleavage to generate a soluble form (3, 5). Cleavage of Epigen by TACE/ADAM17 has been demonstrated (5). The mature, shed form of human Epigen (aa 54 ‑ 104) shares 92%, 94% and 94% aa sequence identity with mouse, rat and equine Epigen, respectively, and more than 40% aa identity with corresponding regions of TGF‑ alpha and epiregulin (2). Epigen mRNA is found in many tissues, but it is mainly expressed in actively proliferating cells within these tissues. For example, Epigen in skin is found mainly in the proliferating root sheath of hair follicles, and transgenic overexpression in the skin causes hyperplasia in sebaceous glands (3, 6). Epigen is also expressed developmentally and by invasive adenocarcinomas of the breast and prostate (3). Epigen is strongly mitogenic for epithelial cells and fibroblasts, despite its relatively weak affinity for its main receptor, ErbB1 (2, 3). The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor‑mediated depletion (3).

References

  1. Schneider, M.R. and E. Wolf (2010) J. Cell. Physiol. 218:460.
  2. Strachan, L. et al. (2001) J. Biol. Chem. 276:18265.
  3. Kochupurakkal, B.S. et al. (2005) J. Biol. Chem. 280:8503.
  4. SwissProt Accession # Q6UW88.
  5. Sahin, U. and C.P. Blobel 2007) FEBS Lett. 581:41.
  6. Dahlhoff, M. et al. (2010) J. Invest. Dermatol. 130:623.

Alternate Names

EPGN

Entrez Gene IDs

255324 (Human); 71920 (Mouse)

Gene Symbol

EPGN

UniProt

Additional Epigen Products

Product Documents for Recombinant Human Epigen Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Epigen Protein

For research use only

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