Product Specifications for Recombinant Human EphA5 Protein, CF
Mouse myeloma cell line, NS0-derived human EphA5 protein Ser56-Pro573, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
75-85 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human EphA5 at 2 µg/mL (100 µL/well) can bind recombinant mouse Ephrin-A2 Fc Chimera with a linear range of 0.16-10 ng/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor tyrosine kinase family which binds members of the Ephrin ligand family (1‑4). The A and B class Eph proteins have a common structural organization. Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. Reverse signaling is propagated through the Ephrin ligand. The human EphA5 cDNA encodes a 1037 amino acid (aa) precursor including a 24 aa signal sequence, a 549 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 443 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Multiple splice variants of human EphA5 are produced in brain and differ in their extracellular and cytoplasmic sequences (6). Taking into account differences in splice variants between species, comparable regions of the human EphA5 ECD share greater than 96% aa sequence identity with bovine, mouse, and rat EphA5, approximately 40% aa identity with human EphA1 and EphA2, and 54‑61% aa identity with human EphA3, 4, 7, and 8. EphA5 is widely expressed in neurons of the adult human CNS (6), with the exception of few areas such as the substantia nigra (7). In rat, the expression of EphA5 during embryogenesis suggests a role in the development of CNS neurons (8).
Poliakov, A. et al. (2004) Dev. Cell 7:465.
Surawska, H. et al. (2004) Cytokine Growth Factor Rev 15:419.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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