Recombinant Human EphA2 Protein, CF
Catalog # 3035-A2 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Gln25-Asn534, with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Immobilized rhEphA2 at 2 µg/mL (100 µL/well) can bind rmEphrin-A1/Fc Chimera with a linear range of 0.078-5 ng/mL.
Formulation, Preparation and Storage
3035-A2
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: EphA2
EphA2, also known as Eck, Myk2, and Sek2, is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5 (1, 2, 3, 4). A and B class Eph proteins have a common structural organization. The human EphA2 cDNA encodes a 976 amino acid (aa) precursor including a 24 aa signal sequence, a 510 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 418 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains (5). The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM) (5). The ECD of human EphA2 shares 90 - 94% aa sequence identity with mouse, bovine, and canine EphA2, and approximately 45% aa sequence identity with human EphA1, 3, 4, 5, 7, and 8. EphA2 becomes autophosphorylated following ligand binding (6, 7) and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways (8, 9). Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin (10), and can be induced by p53 family transcription factors (11). EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells (12, 13). In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels (14). EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration (13). The gene encoding human EphA2 maps to a region on chromosome 1 which is frequently deleted in neuroectodermal tumors (15).
References
- Poliakov, A. et al. (2004) Dev. Cell 7:465.
- Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
- Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
- Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
- Bohme, B et al. (1993) Oncogene 8:2857.
- Pandey, A. et al. (1995) Science 268:567.
- Bartley, T.D. et al. (1994) Nature 368:558.
- Pandey, A. et al. (1994) J. Biol. Chem. 269:30154.
- Miao, H. et al. (2001) Nat. Cell Biol. 3:527.
- Orsulic, S. and R. Kemler (2000) J. Cell Sci. 113:1793.
- Dohn, M. et al. (2001) Oncogene 20:6503.
- Zelinski, D.P. et al. (2001) Cancer Res. 61:2301.
- Brantley, D.M. et al. (2002) Oncogene 21:7011.
- Ogawa, K. et al. (2000) Oncogene 19:6043.
- Sulman, E.P. et al. (1997) Genomics 40:371.
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human EphA2 Protein, CF
Product Specific Notices for Recombinant Human EphA2 Protein, CF
For research use only
Citations for Recombinant Human EphA2 Protein, CF (10)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human EphA2 Protein, CF include:
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Species: N/A
Sample Types: Protein
Applications: ELISA CaptureJH Cha et al. (2022-03-10), Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer The Journal of Biological Chemistry, 2022-00(0):101817.
PMID: 35278434 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayT Fricke et al. (2022-03-05), Antibodies Targeting KSHV gH/gL Reveal Distinct Neutralization Mechanisms Viruses, 2022-014(3).
PMID: 35336948 -
Species: Human
Sample Types: Recombinant Protein
Applications: BioassayL Teo et al. (2021-06-17), Replicating infant-specific reactive astrocyte functions in the injured adult brain Progress in neurobiology, 2021-00(0):102108.
PMID: 34147584 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Mukai et al. (2017-08-29), EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes BMC Cell Biol., 2017-018(1):28.
PMID: 28851287 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Takasugi et al. (2017-06-06), Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2 Nat Commun, 2017-08(0):15729.
PMID: 28585531 -
Species: Yeast
Sample Types: Whole Cells
Applications: Flow CytometryPark S et al. (2015-07-15), Isolation and Characterization of a Monobody with a Fibronectin Domain III Scaffold That Specifically Binds EphA2. PLoS ONE, 2015-010(7):e0132976.
PMID: 26177208 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayLiao-Chan et al. (2015-04-20), Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores. PLoS ONE, 2015-010(4):e0124708.
PMID: 25894652 -
Species: Human
Sample Types: In Vivo,Whole Cells
Applications: In Vivo,NeutralizationHahn AS et al. (2012-06-01), The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus. Nat. Med., 2012-018(6):961-6.
PMID: 22635007 -
Species: Human
Sample Types: In Vivo
Applications: ImmunizationWang QS et al. (2012-03-30), Dietary blue pigments derived from genipin, attenuate inflammation by inhibiting LPS-induced iNOS and COX-2 expression via the NF-kappaB inactivation. PLoS ONE, 2012-07(3):e34122.
PMID: 22479539 -
Sample Types: N/A
Applications: Binding AssayJackson D et al. (2008-11-15), A human antibody-drug conjugate targeting EphA2 inhibits tumor growth in vivo. Cancer Res., 2008-168(22):9367-74.
PMID: 19010911
There are no citations that match your criteria.
Customer Reviews for Recombinant Human EphA2 Protein, CF (2)
Customer Images
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Application: Immunoassay StandardVerified Customer | Posted 07/05/2022works well as an ELISA standard
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Application: Immunoassay StandardVerified Customer | Posted 01/29/2019
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