E. coli-derived human Enolase 2/Neuron-specific Enolase protein Met1-Leu434 with an N-terminal Met and 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
46 kDa, under reducing conditions
Measured by its ability to convert phosphoglyceric acid to phosphoenolpyruvate. The specific activity is >6,000 pmol/min/μg, as measured under the described conditions.
Recombinant Human Enolase 2 His-tag Protein, CF Scientific Data Examples
Recombinant Human Enolase 2 His-tag Protein Binding Activity
2 μg/lane of Recombinant Human Enolase 2/Neuron-specific Enolase His-tag (Catalog # 10412-EN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 46 kDa under reducing conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in MES, NaCl, KCl and MgSO4.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Background: Enolase 2/Neuron-specific Enolase
Neuron specific enolase (NSE), also known as ENO2 or gamma-enolase, is a dimeric, Mg2+-dependent enzyme that catalyzes the dehydration of 2-phospho-D glycate (PGA) to phosphoenolpyruvate (PEP) in the glycolytic pathway and catalyzes the reverse reaction in gluconeogenesis. There are three major isozymes of enolase expressed in selective vertebrate tissues from separate genes: alpha (ENO1), beta (ENO3), and gamma (ENO2). NSE is a highly expressed, specific neuron isozyme (1) making it a useful marker for tumors derived from neuronal cells. Neuron-specific enolase is implicated as a diagnostic and prognostic marker in numerous diseases including early small cell lung cancer (2), prostate cancer (3), multiple myeloma (4), traumatic brain injury (5), acute spinal cord injury (6), acute ischemic stroke (7), and post-concussion symptoms (8). NSE expression and activity are increased in neuronal and glial activation and injury (9), risk factors implicated in neurodegenerative disease. Elevation of NSE promotes glycolysis, proliferation, activation and migration through its C-terminus to activate PI3K and MAPK signal transduction pathways (6, 10) while inhibition of enolase has been shown to attenuate inflammatory events (11, 12). NSE can be regulated through cleavage of the C-termini by cathepsin X (13, 14) or inhibited directly by antibiotic SF2312 (15). Inhibition has been proposed as a therapeutic strategy in cancer (16).
Marangos, P.J. and D.E. Schmechel, (1987) Annu. Rev. Neurosci. 10:269.
Huang, L. et al. (2017) Oncotarget 38:64358.
Muoio, B. et al. (2018) Int. J. Markers 33:10.
Yang, H. et al. (2014) PLoS One. 9:e94304.
Cheng, F. et al. (2014) PLoS One 9:e106680.
Haque, A. et al. (2016) Metab. Brain Dis. 31:487.
Anand, N. et al. (2005) Cerebrovasc. Dis. 20:213.
Mercier, E. et al. (2018) Brain Inj. 32:29.
Isgro, M.A. et al. (2015) Adv. Exp. Med. Biol. 867:125.
Hafner, A. et al. (2013) Aging Cell 12:604.
Polcyn, R. et al. (2017) J. Clin. Cell. Immunol. 8:536.
Haque, A. et al. (2017) Neurochem. Res. 42:2777.
Obermajer, N. et al. (2009) Int. J. Biochem. Cell Biol. 41:1685.
Hafner, A. et al. (2012) Biochem. J. 443:439.
Leonard, P.G. et al. (2016) Nat. Chem. Bio. 12:1053.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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