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Recombinant Human Endoglycan/PODXL2 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1524-EG

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1524-EG-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Endoglycan/PODXL2 protein
Gly33-Thr500, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly33

Predicted Molecular Mass

52 kDa

SDS-PAGE

85-110 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.
The ED50 for this effect is 0.1-0.6 μg/mL.

Formulation, Preparation and Storage

1524-EG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglycan/PODXL2

Human Endoglycan, also called Podocalyxin-like 2 protein (PODXL2), is an extensively O-glycosylated type I transmembrane protein in the CD34 family of sialomucins (1, 2). Mature human Endoglycan consists of a 468 amino acid (aa) extracellular domain that contains an N-terminal acidic domain characterized by several polyglutamate tracts, a mucin domain, and a membrane-proximal globular domain. Within the extracellular domain, human Endoglycan shares 74% amino acid identity with both mouse and rat Endoglycan. Alternative splicing may generate a short isoform that lacks aa 336-411. Following the extracellular domain is a single transmembrane spanning helix and a charged cytoplasmic tail that contains a C-terminal PDZ-domain binding motif (1, 3). The cytoplasmic domain of Endoglycan has been shown to bind to Na+/H+ exchanger regulatory factor-1 (NHERF-1), which acts as a scaffolding protein that links membrane proteins to the actin cytoskeleton (4). Human Endoglycan exists as a disulfide-linked homodimer that is expressed in vascular endothelium, smooth muscle, hematopoietic precursor populations and leukocyte subpopulations (1, 5). Endoglycan functions through its interactions with selectins and is involved in leukocyte-endothelial cell adhesion although depending on cellular context and post-translational modifications, Endoglycan may also demonstrate anti-adhesive properties (3, 5).

 

References

  1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
  2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
  3. Nielsen, J.S. & K.M. McNagny (2008) J. Cell Sci. 121:3683.
  4. Tan, P.C. et al. (2006) Stem Cells 24:1150.
  5. Kerr, S.C. et al. (2008) J. Immunol. 181:1480.
  6.  

Alternate Names

PODLX2, PODXL2

Entrez Gene IDs

50512 (Human); 319655 (Mouse)

Gene Symbol

PODXL2

UniProt

Additional Endoglycan/PODXL2 Products

Product Documents for Recombinant Human Endoglycan/PODXL2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Endoglycan/PODXL2 Protein, CF

For research use only

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