Product Specifications for Recombinant Human Endoglycan/PODXL2 Protein, CF
Mouse myeloma cell line, NS0-derived human Endoglycan/PODXL2 protein Gly33-Thr500, with a C-terminal 10-His tag
>95%, by SDS-PAGE with silver staining
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
85-110 kDa, reducing conditions
Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is 0.1-0.6 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Endoglycan, also called Podocalyxin-like 2 protein (PODXL2), is an extensively O-glycosylated type I transmembrane protein in the CD34 family of sialomucins (1, 2). Mature human Endoglycan consists of a 468 amino acid (aa) extracellular domain that contains an N-terminal acidic domain characterized by several polyglutamate tracts, a mucin domain, and a membrane-proximal globular domain. Within the extracellular domain, human Endoglycan shares 74% amino acid identity with both mouse and rat Endoglycan. Alternative splicing may generate a short isoform that lacks aa 336-411. Following the extracellular domain is a single transmembrane spanning helix and a charged cytoplasmic tail that contains a C-terminal PDZ-domain binding motif (1, 3). The cytoplasmic domain of Endoglycan has been shown to bind to Na+/H+ exchanger regulatory factor-1 (NHERF-1), which acts as a scaffolding protein that links membrane proteins to the actin cytoskeleton (4). Human Endoglycan exists as a disulfide-linked homodimer that is expressed in vascular endothelium, smooth muscle, hematopoietic precursor populations and leukocyte subpopulations (1, 5). Endoglycan functions through its interactions with selectins and is involved in leukocyte-endothelial cell adhesion although depending on cellular context and post-translational modifications, Endoglycan may also demonstrate anti-adhesive properties (3, 5).
Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
Nielsen, J.S. & K.M. McNagny (2008) J. Cell Sci. 121:3683.
Tan, P.C. et al. (2006) Stem Cells 24:1150.
Kerr, S.C. et al. (2008) J. Immunol. 181:1480.
Entrez Gene IDs
50512 (Human); 319655 (Mouse)
Endoglycan, PODLX2, PODXL2, podocalyxin-like 2, podocalyxin-like protein 2
Citations for Recombinant Human Endoglycan/PODXL2 Protein, CF
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Product Documents for Recombinant Human Endoglycan/PODXL2 Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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