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Recombinant Human Embigin/EMB Fc Chimera Protein CF

Catalog # 10832-EM | R&D Systems, Inc. a Bio-Techne Brand
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10832-EM-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Embigin/EMB protein
Human Embigin/EMB
(Asp33-Pro260)
Accession # Q6PCB8.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp33

Predicted Molecular Mass

52 kDa

SDS-PAGE

85-100 kDa, under reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Embigin/EMB Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Human Embigin/EMB Fc Chimera Protein CF

Recombinant Human Embigin/EMB Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human Embigin/EMB Fc Chimera (Catalog # 10832-EM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-100 kDa and 170-200 kDa, respectively.

Formulation, Preparation and Storage

10832-EM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Embigin/EMB

Embigin (EMB) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily (IgSF) proteins. It is a cell adhesion molecule that is preferentially expressed during mouse embryogenesis (1). Embigin, along with basigin and neuroplastin, form a small subgroup within the IgSF, with all members possessing a glutamate at the same position in the transmembrane domain (2). Mature, human Embigin is highly glycosylated and consists of an extracellular domain (ECD) wjth two Ig domains, a transmembrane region, and a short intracellular region. Within the ECD, human EMB shares 75% and 62% amino acid identity with mouse and rat EMB, respectively. Embigin is an accessory protein of monocarboxylic acid transporter, MCT2 which participates in transporting lactic acid between glial cells and neurons (3). Thus, it may be related to brain energy metabolism and has been investigated as a susceptible gene for schizophrenia (4). Embigin is highly expressed in early embryos of mice, and in the heart, lung, brain and other tissues of adult rats (5, 6). Loss of embigin promotes proliferation, anchorage-independent growth, and migration ability of breast cancer cells (7).

References

  1. Fan, Q.W. et al. (1998) Dev. Growth Differ. 40:277.
  2. Muramatsu, T, and Miyauchi, T. (2003) Histol. Histopathol. 18:981.
  3. Perez-Escuredo, J. et al. (2016) Biochim. Biophys. Acta 863:2481.
  4. Zhou, J. et al. (2020) BMC Psychiatry 20:135.
  5. Huang, R. et al. (1990) Differentiation 45:76.
  6. Guenette, R. et al. (1997) Dev. Genet. 4:268.
  7. Chao, F. et al. (2015) Oncotarget 6:23496.

Alternate Names

EMB, GP70

Entrez Gene IDs

133418 (Human); 13723 (Mouse); 114511 (Rat)

Gene Symbol

EMB

UniProt

Product Documents for Recombinant Human Embigin/EMB Fc Chimera Protein CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Embigin/EMB Fc Chimera Protein CF

For research use only

Reconstitution Calculator

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