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Recombinant Human ELFN2 Fc Chimera Protein, CF

Catalog # 10643-EF | R&D Systems, Inc. a Bio-Techne Brand
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10643-EF-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ELFN2 protein
Human ELFN2
(Asp23-Tyr397)
Accession # Q5R3F8.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp23

Predicted Molecular Mass

69 kDa

SDS-PAGE

97-112 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human mGluR7 Protein (Catalog # 9530-UR) is coated at 1 μg/mL, 100 μL/well, Recombinant Human ELFN2 Fc Chimera binds with an ED50 of 0.15-0.75 μg/mL.

Scientific Data Images for Recombinant Human ELFN2 Fc Chimera Protein, CF

Recombinant Human ELFN2 Fc Chimera Protein Binding Activity.

When Recombinant Human mGluR7 Protein (9530-UR) is coated at 1 μg/mL, 100 μL/well, Recombinant Human ELFN2 Fc Chimera binds with an ED50 of 0.15-0.75 μg/mL.

Recombinant Human ELFN2 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human ELFN2 Fc Chimera Protein (Catalog # 10643-EF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 97-112 kDa and 194-224 kDa, respectively.

Formulation, Preparation and Storage

10643-EF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ELFN2

Extracellular leucine-rich repeat and fibronectin type III domain-containing protein 2 (ELFN2), also known as protein phosphatase 1 regulatory subunit 29 (PPP1R29), is a member of the extracellular leucine-rich repeat (LRR) superfamily (1). Human ELFN2 consists of an extracellular domain (ECD) containing five LRR domains, one LRR C-terminal (LRRCT) domain and an FN3 domain, a transmembrane domain, and a cytoplasmic domain. Within the mature ECD, human ELFN2 shares 98% amino acid sequence identity with mouse and rat ELFN2. ELFN-2 is quite restricted to the nervous system in which it is broadly expressed in the cortex,  presumed glutamatergic neurons, hippocampus, pyramidal and granule cells, and in the striatum. It has been proposed that ELFN-2 could provide the requisite specificity of cellular interactions to mediate a large number of selective connectivity decisions thereby involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (2). ELFN2 has been identified as a novel postsynaptic adhesion molecule expressed throughout the brain (3). Mutations of ELFN2 have been reported in pancreatic cancer radiation resistance (4) and stress fracture (5).



References

  1. Liu, C. et al. (2018) Mol. Ther. 26:2267.
  2. Dolan, J. et al. (2007) BMC Genomics 8:320.
  3. Dunn, H.A. et al. (2019) Mol. Psychiatry 24:1902.
  4. Friedman E. et al. (2014) Genet. Res. (Camb) 96:e004.
  5. Souchek L.L. et al. (2014) Br. J. Cancer 111:1139.

Long Name

Extracellular Leucine-Rich Repeat And Fibronectin Type III Domain Containing 2

Alternate Names

LRRC62, PPP1R29

Entrez Gene IDs

114794 (Human); 207393 (Mouse)

Gene Symbol

ELFN2

UniProt

Product Documents for Recombinant Human ELFN2 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ELFN2 Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

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