Recombinant Human EGFR His-tag Protein, CF New

Name: Recombinant Human EGFR His-tag Protein, CF
Brand: R&D Systems
SKU: 11142-ER

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11142-ER

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	P00533.2
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human EGFR His-tag Protein, CF

Product Specifications for Recombinant Human EGFR His-tag Protein, CF

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived human EGFR protein Gly696-Gly1022, with an N-terminal Met and 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	38 kDa
SDS-PAGE	39 kDa, under reducing conditions
Activity	Measured by its ability to transfer phosphate from adenosine triphosphate (ATP) to a synthetic peptide substrate. The specific activity is >35 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human EGFR His-tag Protein, CF

	Recombinant Human EGFR His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human EGFR His-tag Protein (Catalog # 11142-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11142-ER

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular ligand binding domain containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1,2). The human EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain (3,4). The kinase domain contains the conserved catalytic domain with an ATP binding site in the cleft of two lobes at the N-terminal and C-terminal regions and an activation loop with a DFG motif that changes conformation to regulate activity (5-7). EGFR binds a subset of the EGF family ligands (1,2) that induce EGFR homodimerization as well as heterodimerization with other family members resulting in kinase activation and cell signaling (8-10). EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (11,12). EGFR has been reported as overexpressed and/or mutated in tumors with high levels of activation of EGFR related to poor prognosis and tumor regression rates (13,14). EGFR kinase inhibition is a promising target for cancer therapeutics and includes development of drugs to address EGFR-mutation conferred resistance (7,15).

Item	Value
References	Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183. Shilo, B.Z. (2005) Development 132:4017. Lin, C. et al. (1984) Science 224:843. Ullrich, A. et al. (1984) Nature 309:418. Stamos, J. et. al. (2002) J. Biol. Chem. 277:46265. Liu, Q. et al. (2013) Chem. Biol. 20:146. Amelia, T. et al. (2022) Molecules 27:819. Graus-Porta, D. et al. (1997) EMBO J. 16:1647. Yarden, Y. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:127. Lemmon, M.A. (2009) Exp. Cell Res. 315:638. Sibilia, M. and E.F. Wagner (1995) Science 269:234. Miettinen, P.J. et al. (1995) Nature 376:337. Janne, P.A. et al. (2005) J. Clin. Oncol. 23:3227. Yun, C.-H. et al. (2008) Proc. Nat. Acad. Sci. USA 105:2070. Yu, J. et al. (2022) Front. Mol. Biosci. 9:847825.
Long Name	Epidermal Growth Factor Receptor
Entrez Gene IDs	1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names	EC 2.7.10, EC 2.7.10.1, EGF R, EGFR, ERBB1PIG61, ErbB, ErbB1, HER-1, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), mENA

Citations for Recombinant Human EGFR His-tag Protein, CF

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant Human EGFR His-tag Protein, CF New

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Product Specifications for Recombinant Human EGFR His-tag Protein, CF

Scientific Data Examples for Recombinant Human EGFR His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: EGFR

Citations for Recombinant Human EGFR His-tag Protein, CF

Product Documents for Recombinant Human EGFR His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected