Recombinant Human EGF GMP Protein, CF
R&D Systems, Inc. a Bio-Techne Brand
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Citations for Recombinant Human EGF GMP Protein, CF (1)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human EGF GMP Protein, CF include:
Sample Types: Whole Cells
Applications: BioassayCJ Brereton et al. (2022), Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis Elife, 11(0).
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Customer Reviews for Recombinant Human EGF GMP Protein, CF (2)
Application: CellProlifVerified Customer | Posted 08/17/2021EGF-GMP used to convert fibroblast into organoid in GMP condition.
Application: CellProlifVerified Customer | Posted 07/21/20210.2ng/ml EGF was added to promote the growth of the limbal stem cells
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FAQs for Recombinant Human EGF GMP Protein, CF
The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?
Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.
What is the recommended method for reconstitution of a lyophilized protein or antibody?
Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand. Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.