Recombinant Human EGF, Animal-Free Protein
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Source: | E. coli |
Accession #: | P01133 |
Applications: | Bioactivity |
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Citations for Recombinant Human EGF, Animal-Free Protein (5)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human EGF, Animal-Free Protein include:
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Species: Human
Sample Types: Whole Tissue
Applications: Cell CultureS Ding et al. (2022), Patient-derived micro-organospheres enable clinical precision oncology Cell Stem Cell, 0(0).
PMID: 35508177 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Berlak et al. (2022), Mutations in ALK signaling pathways conferring resistance to ALK inhibitor treatment lead to collateral vulnerabilities in neuroblastoma cells Molecular Cancer, 21(1):126.
PMID: 35689207 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureMJ Baker et al. (2020), Evaluation of active Rac1 levels in cancer cells: a case of misleading conclusions from immunofluorescence analysis J. Biol. Chem., 0(0).
PMID: 32817335 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayMJ Baker et al. (2020), Evaluation of active Rac1 levels in cancer cells: a case of misleading conclusions from immunofluorescence analysis J. Biol. Chem., 0(0).
PMID: 32817335 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayYH Cui et al. (2018), FBXL14 abolishes breast cancer progression by targeting CDCP1 for proteasomal degradation Oncogene, 0(0).
PMID: 29973690
There are no citations that match your criteria.
FAQs for Recombinant Human EGF, Animal-Free Protein
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What is the recommended method for reconstitution of a lyophilized protein or antibody?
Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand. Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.
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Are R&D Systems recombinant proteins and antibodies sterile?
Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.