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Recombinant Human EGF, Animal-Free Protein

R&D Systems, Inc. a Bio-Techne Brand

Recombinant Human EGF, Animal-Free Protein
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AFL236-200
AFL236
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Setting the standard in quality research reagents

Key Product Details

Source: E. coli
Accession #: P01133
Applications: Bioactivity

Citations for Recombinant Human EGF, Animal-Free Protein (5)

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FAQs for Recombinant Human EGF, Animal-Free Protein

  • What is the recommended method for reconstitution of a lyophilized protein or antibody?

    Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.   

  • Are R&D Systems recombinant proteins and antibodies sterile?

    Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Product Documents for Recombinant Human EGF, Animal-Free Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.