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Recombinant Human EDA-A2 Protein

Bio-Techne includes R&D Systems | Catalog # 922-ED

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Carrier Free
922-ED-010/CF

Catalog #
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With Carrier
922-ED-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human EDA-A2/Ectodysplasin A2 protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Human EDA-A2
(Ala179-Ser389)
Accession # AAC77371
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

24.7 kDa

SDS-PAGE

28-38 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera (Catalog # 1093-XD) is coated at 0.1  μg/mL (100 μL/well), the concentration of Recombinant Human EDA-A2/Ectodysplasin A2 that produces 50% of the optimal binding response is 3-15 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 922-ED
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 922-ED/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDA-A2/Ectodysplasin A2

EDA-A2, a product of the EDA gene (also called Tabby), is a type II transmembrane protein that is a member of the TNF Superfamily (TNFSF). Human EDA-A2 is a 389 amino acid (aa) protein with a predicted N-terminal 39 aa cytoplasmic domain, a 22 aa transmembrane domain and a C-terminal 328 aa extracellular domain. The extracellular domains of human and mouse EDA-A2 share approximately 94% identity. Within the TNFSF, EDA-A2 shares the highest homology with EDA-A1, the other product of the EDA gene. EDA-A2 and EDA-A1 are splice variants of EDA which differ by only two amino acids. EDA-A1 contains two additional amino acids, Glu 308 and Val 309. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor Superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF family member with high homology to EDAR. Mutations in EDA-A1, EDA-A2, EDAR and XEDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene.

References

  1. Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661.
  2. Kere, J. et al. (1996) Nat. Genet. 13:409.
  3. Monreal, A.W. et al. (1998) Am. J. Hum. Genet. 63:380.
  4. Headon, D.J. and P.A. Overbeek (1999) Nat. Genet. 22(4):370.
  5. Kumar, A. et al. (2000) J. Biol. Chem. 276(4):2668.
  6. Monreal, A.W. et al. (1999) Nat. Genet. 22(4):366.
  7. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94(24):13069.
  8. Yan, M. et al. (2000) Science 290(5491):523.

Alternate Names

EDAA2

Entrez Gene IDs

1896 (Human); 13607 (Mouse)

Gene Symbol

EDA

UniProt

Additional EDA-A2/Ectodysplasin A2 Products

Product Documents for Recombinant Human EDA-A2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human EDA-A2 Protein

For research use only

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