Chinese Hamster Ovary cell line, CHO-derived human EBI3 protein Arg21-Lys220
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
30-40 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (Catalog # 7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.
Recombinant Human EBI3 Protein, CF Scientific Data Examples
Recombinant Human EBI3 Protein Binding Activity.
Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.
Recombinant Human EBI3 Protein SDS-PAGE.
2 μg/lane of Recombinant Human EBI3 Protein (Catalog # 11084-EI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-40 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
EBI3 (Epstein-Barr virus-induced gene 3 protein) also known as IL-27B, is a secreted cytokine (1). It shares similar domain structure as IL-12B except there are only two fibronectin type -III domains instead of three. Through non-covalent interaction, EBI-3 binds to IL-12A (IL-12 p35), IL-23A (IL-23p19), or IL-27A (IL-27p28/IL-30) to form IL-35, IL-39, or IL-27, respectively (2-4). In addition to forming the heterodimeric cytokines, EBI-3 can also function on its own. Expression of EBI-3 is induced in neutrophils stimulated with TLR8 ligands, with no detectable increase in IL-35, IL-39 or IL-27 (5). EBI-3 also mediate IL-6 trans-signaling through receptor gp130 in B9 mouse plasmacytoma cells (6).
Devergne, O. et al. (1996) J. Virol. 70:1143.
Devergne, O. et al. (1997) Protc. Natl. Acad. Sci. U.S.A. 94:12041.
Pflanz, S. et al. (2002) Immunity 16:779.
Wang, X. et al. (2016) Eur. J. Immuno. 46:1343.
Cassatella, M.A. et al. (2020) J Leukoc. Biol. 108:1515.
Chehboun, S. et al. (2017) J Biol. Chem. 292:6644.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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