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Recombinant Human DLL1 His-tag Protein, CF

Catalog # 1818-DL | R&D Systems, Inc. a Bio-Techne Brand
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1818-DL-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human DLL1 protein
Human DLL1
(Ser22-Gly540) &
(His152-Gly540)
Accession # AAG09716
DI 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22 & His152

Predicted Molecular Mass

57 kDa and 43 kDa

SDS-PAGE

66 kDa and 55 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842.
The ED50 for this effect is 0.25-1.0 µg/mL.

Formulation, Preparation and Storage

1818-DL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90 - 100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma-secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6 - 11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

References

  1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 
  3. Karanu, F.N. et al. (2001) Blood 97:1960. 
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 
  6. Takahashi, Y. et al. (2003) Development 130:4259. 
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
  12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.

Long Name

Delta-like 1

Alternate Names

Delta 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Gene Symbol

DLL1

UniProt

Product Documents for Recombinant Human DLL1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human DLL1 His-tag Protein, CF

For research use only

Citations for Recombinant Human DLL1 His-tag Protein, CF (8)

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FAQs for Recombinant Human DLL1 His-tag Protein, CF

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  • Q: What is the molecular weight (MW) of your Recombinant Human DLL1 His-tag Protein?

    A: Our Recombinant Human DLL1 His-tag Protein contains both a full-length isoform and a truncated isoform. The sequence of the full length isoform is Ser22-Gly540, with a predicted MW of 57 kDa. The sequence of the truncated isoform is His152-Gly540, with a predicted MW of 43 kDa.

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