Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF

Name: Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF
Brand: R&D Systems
SKU: 1934-DP
Price: 417 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1934-DP

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	Q12913
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF

Product Specifications for Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF

Source	E. coli-derived human DEP-1/CD148 protein Arg997-Ala1337, with an N-terminal Met & 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	40 kDa
SDS-PAGE	37 kDa, reducing conditions
Activity	Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006). The specific activity is >100 µmol/min/mg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1934-DP

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and Betamercaptoethanol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kilodalton) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta‑catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

Item	Value
References	Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680. Sorby, M. et al. (2001) Oncogene 20:5219. Jandt, E. et al. (2003) Oncogene 22:4175. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393. Borges, L.G. et al. (1996) Circ. Res. 79:570. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219. Holsinger, L.J. et al. (2002) Oncogene 21:7067.
Long Name	Density Enhanced Receptor Protein Tyrosine Phosphatase
Entrez Gene IDs	5795 (Human); 19271 (Mouse); 29645 (Rat)
Alternate Names	CD148, CD148 antigen, DEP-1, DEP1, DEP1EC 3.1.3.48, Density-enhanced phosphatase 1, HPTP eta, HPTP-eta, HPTPeta, PTPRJ, Protein-tyrosine phosphatase eta, Protein-tyrosine phosphatase receptor type J, R-PTP-ETA, R-PTP-J, SCC1, human density enhanced phosphatase-1, protein tyrosine phosphatase, receptor type, J, protein tyrosine phosphatase, receptor type, J polypeptide, receptor-type tyrosine-protein phosphatase eta, susceptibility to colon cancer 1, mouse, homolog of

Citations for Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: DEP-1/CD148

Citations for Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF

Product Documents for Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected