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Recombinant Human DDR1 Fc Chimera Protein, CF

Catalog # 2396-DR | R&D Systems, Inc. a Bio-Techne Brand
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2396-DR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human DDR1 protein
Human DDR1
(Asp21 - Thr416)
Accession # Q5ST11
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp21

Predicted Molecular Mass

70.5 kDa (monomer)

SDS-PAGE

90-95 kDa, reducing conditions

Activity

Measured by its ability to bind Collagen I in a functional ELISA. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind rhDDR1 with an apparent KD <10 nM.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

2396-DR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDR1

DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120 - 140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1, 2). Mature human DDR2 consists of a 398 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 27 aa transmembrane segment, and a 470 aa cytoplasmic region with a tyrosine kinase domain (3). Within the ECD, human DDR1 shares 53% aa sequence identity with human DDR2 and 93% with mouse and rat DDR1. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers (2, 4). Compared to isoform DDR1b, DDR1a lacks 37 aa’s that include a Shc-interacting NPxY motif in the cytoplasmic juxtamembrane region (5). Two additional kinase deficient splice forms are expressed in colon cancer (6). The discoidin-like domain mediates binding to collagens I - V (1, 7, 8). DDR1 selectively recognizes the triple helical structure of collagen (7, 8). It is expressed on the cell surface as a dimer which can include different isoforms (5, 9). DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis (10, 11). The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization (9). Collagen binding induces prolonged autophosphorylation, including the NPxY motif (7, 8). Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment (12, 13). TIMP3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF (12). Overexpression of DDR1a promotes MMP-2 activation and results in an increased invasiveness of a glioblastoma cell line; DDR1b does not (14).

References

  1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
  2. Yoshimura, T. et al. (2005) Immunologic Res. 31:219.
  3. Perez, J.L. et al. (1994) Oncogene 9:211.
  4. Laval, S. et al. (1994) Cell Growth Differ. 5:1173.
  5. Perez, J.L. et al. (1996) Oncogene 12:1469.
  6. Alves, F. et al. (2001) FASEB J. 15:1321.
  7. Shrivastava, A. et al. (1997) Mol. Cell 1:25.
  8. Vogel, W. et al. (1997) Mol. Cell 1:13.
  9. Nordeen, N.A. et al. (2006) J. Biol. Chem. 281:22744.
  10. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
  11. Agarwal, G. et al. (2007) J. Mol. Biol. 367:443.
  12. Slack, B.E. et al. (2006) J. Cell Biochem. 98:672.
  13. Vogel, W.F. et al. (2001) FEBS Lett. 514:175.
  14. Ram, R. et al. (2006) J. Neurooncol. 76:239.

Long Name

Discoidin Domain Receptor 1

Alternate Names

CAK, CD167a, RTK6, TrkE

Entrez Gene IDs

780 (Human)

Gene Symbol

DDR1

UniProt

Product Documents for Recombinant Human DDR1 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human DDR1 Fc Chimera Protein, CF

For research use only

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