Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

Name: Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 142-DC
Price: 428 USD

Catalog # 142-DC | R&D Systems, Inc. a Bio-Techne Brand

Citations (11)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	O95407
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

Product Specifications for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DcR3/TNFRSF6B protein

Human DcR3 (Val 24-His300) Accession # O95407	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Val24

Predicted Molecular Mass

57 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED₅₀ for this effect is 0.03‑0.15 µg/mL in the presence of 20 ng/mL rhFas Ligand.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

142-DC

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DcR3/TNFRSF6B

Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.

Item	Value
References	Pitti, R.M. et al. (1998) Nature 396:699. Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733. Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.
Long Name	Decoy Receptor 3
Entrez Gene IDs	8771 (Human); 107126607 (Cynomolgus Monkey)
Alternate Names	DCR3Decoy receptor for Fas ligand, DcR3, Decoy receptor 3, M68, M68DJ583P15.1.1, TNFRSF6B, TR6, TR6DcR3, tumor necrosis factor receptor superfamily member 6B, tumor necrosis factor receptor superfamily, member 6b, decoy

Citations for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF (11)

Showing 1 - 10 of 11 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Zwolak et al. (2022-11-29), A stable, engineered TL1A ligand co-stimulates T cells via specific binding to DR3 Scientific Reports, 2022-112(1):20538.
PMID: 36446890
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

YJ Peng et al. (2020-06-10), Decoy Receptor 3 Promotes Preosteoclast Cell Death via Reactive Oxygen Species-Induced Fas Ligand Expression and the IL-1alpha/IL-1 Receptor Antagonist Pathway Mediators Inflamm., 2020-02020(0):1237281.
PMID: 32587467
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Idelson et al. (2018-08-16), Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Stem Cell Reports, 2018-00(0).
PMID: 30122442
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Capture

AW Clarke et al. (2018-03-05), An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018-00(0):1-43.
PMID: 29436901
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012-05-14), DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1. BMC Cancer, 2012-012(0):176.
PMID: 22583667
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tso GH et al. (2010-05-01), Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells. Stem Cells, 2010-028(5):939-54.
PMID: 20222014
Sample Types: Virus
Applications: Surface Plasmon Resonance

Morishige T et al. (2010-02-01), Creation of a LIGHT mutant with the capacity to evade the decoy receptor for cancer therapy. Biomaterials, 2010-031(12):3357-63.
PMID: 20117833
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Colucci S et al. (2009-07-09), Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients. Leukemia, 2009-023(11):2139-46.
PMID: 19587706
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Mueller AM et al. (2009-03-06), The Decoy Receptor 3 (DcR3, TNFRSF6B) suppresses Th17 immune responses and is abundant in human cerebrospinal fluid. J. Neuroimmunol., 2009-0209(1):57-64.
PMID: 19269042
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Nakamichi Y et al. (2007-01-01), Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts. J. Immunol., 2007-0178(1):192-200.
PMID: 17182555
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Edwards JR et al. (2006-05-01), LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis. Arthritis Rheum., 2006-054(5):1451-62.
PMID: 16649193

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Product Documents for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

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Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

Catalog # 142-DC | R&D Systems, Inc. a Bio-Techne Brand

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