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Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF

Catalog # 162-D2 | R&D Systems, Inc. a Bio-Techne Brand
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162-D2-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human DC-SIGNR/CD299 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human DC-SIGNR
(Ser73-Glu376)
Accession # NP_001138382
N-terminus C-terminus

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

61.4 kDa (monomer)

SDS-PAGE

61-66 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells.
When 5 x 104 cells/well are added to rhDC-SIGNR/Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately 40%-70% of added cells will adhere after 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

162-D2
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-SIGNR/CD299

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).

References

  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2

Alternate Names

CD209L, CD299, CLEC4M, DC-SIGN2, DCSIGNR, LSIGN

Entrez Gene IDs

10332 (Human)

Gene Symbol

CLEC4M

UniProt

Product Documents for Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF (6)

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